摘要
目的:测定百合知母汤的水煎液中总皂苷和总黄酮含量。方法:利用紫外分光光度UV法对百合知母汤的总皂苷和总黄酮来测定。结果:菝葜皂苷元线性关系为y=0.024 5x+0.016,加样回收率为97.12%,RSD=2.81%。芒果苷线性关系为y=0.070 1x-0.118 8,加样回收率为98.56%,RSD=1.64%。结论:此方法准确、快速、简便,适合于百合知母汤中总皂苷和总黄酮含量的测定,可以作为该方剂的一种质量控制方法。
Objective:To measure the contents of total saponins and total flavonoids in Baihe ZhimuDecoction. Method:The UV method was used to determine the contents of total saponins and total flavonoids in Baihe Zhimu Decoction. Result:The Sarsasapogenin linear relation was y=0.024 5x+0.016 and the recovery rate was 97.12%,RSD=2.81%. Mangiferin linear relation was y=0.070 1x-0.118 8 and the recovery rate was 98.56%,RSD=1.64%. Conclusion:This method is indeed rapid,simple and suitable for contents determination of total saponins and total flavonoids in Baihe Zhimu Decoction,which can be the quality control method.
出处
《辽宁中医药大学学报》
CAS
2015年第2期54-56,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
知母
百合
总黄酮
总皂苷
含量测定
anemarrhena rhizome
lily bulb
total flavonoid
total saponins
content determination