壳聚糖中药膜的制备及其薄层色谱鉴别

Preparation and TLC of Chitosan Chinese Medicine Membrane

目的:制备壳聚糖中药膜并建立其薄层色谱鉴别方法。方法:以壳聚糖为成膜载体,黄柏、黄芪、大黄、白及为主要成分,制备壳聚糖中药膜。并采用薄层色谱法(TLC),对本制剂中的黄柏、黄芪、大黄进行鉴别研究,以其各自的有效成分及阴性对照品进行对照实验。结果:经过多次鉴别实验后,TLC斑点清晰,分离度好,具有良好的重现性,且阴性无干扰。结论:该膜剂处方组成合理,制备工艺简单。用薄层色谱法鉴别壳聚糖中药膜的实验具有操作简单、稳定、快速、准确、专属性强的特点,所建方法可用于壳聚糖中药膜质量控制的定性依据,为今后标准的进一步修订打下了基础。

Objective：To work out the preparation of chitosan Chinese medicine membrane and to establish a thin layer distinction method. Methods：The chitosan was as a carrier membrane,choosing Cortex Phellodendri Chinensis,Astragalus,Rhubarb,Common Bletilla Tuber as the main ingredients for the preparation of chitosan Chinese medicine membrane. The thin layer chromatography（TLC）method was used to make the identification studies on Cortex Phellodendri Chinensis,Astragalus and Rhubarb of this medicine and implied contrast tests of their active ingredients and its negative controls. Results：After several identification experiments,TLC spots were clear and presented good resolution with accepted reproducibility,negative with non-interference. Conclusion：The membrane has a reasonable forming prescription and the preparation process is simple. Identification by thin-layer chromatography experiments,chitosan Chinese medicine membrane is simple,stable,fast,accurate and specific. It presents strong features. The method can be used to build as the qualitative basis of chitosan membrane quality control of traditional Chinese medicine,which also can set up the foundation for further amendments for future.