毛尖紫萼藓类泛素蛋白基因的克隆与表达分析

Cloning and Expression Analysis of Ubiquitin-like Protien Gene in Grimmia pilifera

为研究类泛素蛋白基因在苔藓植物中的生物学功能,从毛尖紫萼藓干旱c DNA文库中获得了类泛素蛋白基因c DNA全长序列,命名为Gp-UBL5。该基因c DNA全长471 bp,包含1个222 bp的完整开放阅读框,编码73个氨基酸组成的蛋白。生物信息学分析表明,Gp-UBL5基因编码蛋白分子质量为8.525 k Da,等电点为7.84,为稳定型蛋白。进化分析显示,该编码蛋白与二穗短柄草、大麦等类泛素蛋白具有较高一致性,达96%-99%。实时荧光定量PCR结果表明,Gp-UBL5基因在复水和快速干旱的各个时期均有表达,推测该基因可能在毛尖紫萼藓的抗旱机制中发挥作用。

In order to study biological function of ubiquitin-like protein（ UBL） in bryophytes,A full-length c DNA sequence of ubiquitin-like protein（ UBL） gene was obtained from Grimmia pilifera c DNA library,named GpUBL5. The full length of Gp-UBL5 was 471 bp,contained a 222 bp open reading frame（ ORF）,and encoding a protein with 73 amino acids. Bioinformatics analysis suggested that this protein was a stable protein with a calculated molecular mass of 8. 525 k Da and a theoretical p I of 7. 84. Phylogenetic analysis indicated that Gp-UBL5 protein had 96% to 99% identities with other UBL5 proteins from different species,such as Brachypodium distachyon and Hordeum vulgare. The qRT-PCR result showed that Gp-UBL5 gene could express in rehydration and dehydration. It was speculated that the gene might play an important role in response to drought. These results provided important information for the further study of functional verification.