110份普通玉米自交系的SSR聚类分析

Cluster Analysis in 110 Ordinary Maize Inbred Lines Revealed by SSR Markers

为了明确110份玉米自交系的亲缘关系,更好地利用和改良玉米自交系,以代表国内主要杂种优势群的4个标准测验种（丹340、黄早4、Mo17、K12）与106份普通玉米自交系为材料,利用SSR分子标记进行遗传多样性分析,选用28对SSR引物对供试材料进行了遗传多样性研究。结果表明,在供试材料中共检测到125个等位基因变异,平均每对引物检测到的等位基因变异为4.46个,变异为2-10个,平均多态性信息量PIC值为0.85,变化范围为0.75-0.94。利用UPGMA聚类分析方法可将110份普通玉米自交系划分为6个类群。

In order to specify the genetic relationship of 110 maize inbred lines,better use of and improve maize inbred lines,four key inbred lines representing 4 heterotic groups（ Dan 340,Huangzao 4,Mo17,K12） were used dominantly in China and 106 ordinary maize inbred lines were selected for the genetic diversity analysis by SSR Markers 28 SSR primers were selected. The results showed that 125 alleles were detected in there inbred lines,bands,an average of 4. 46 alleles per primer,variation of 2- 10 alleles for each primer,mean polymorphic information content of 0. 85 and its range from 0. 75 to 0. 94 were detected. The 110 inbred lines were divided into 6 groups by UPGMA method.