不同品种(系)烤烟西柏三烯二醇代谢差异研究

Metabolism differences of cembratrien-diols in different tobacco cultivars

为阐明烟草西柏三烯二醇代谢机理,采用GC-MS测定了8306、12451、云烟87、Y892×8306、豫烟11号、NX232和中烟100,7个烤烟品种(系)中部叶龄60 d叶面分泌物组分,并对其中具有代表性的4个材料的西柏三烯二醇合成关键酶基因CYC-1和CYP71D16进行了荧光定量PCR测定,对生育期叶面分泌物的变化、调制后烟叶茄酮含量和感官质量进行了分析评价。结果表明,7个烤烟品种(系)中部叶叶龄60 d时烟叶的叶面分泌物中,共测定出13种非挥发性化合物,主要成分为西柏三烯二醇;8306具有最高的叶面分泌物和西柏三烯二醇含量,豫烟11号次之,12451最低;CYC-1和CYP71D16在8306中的相对表达量显著高于12451和云烟87,初步说明这两个基因的上调表达促进了西柏三烯二醇的合成。成熟期具有较大西柏三烯二醇降解量的8306和豫烟11号在调制后拥有更高的茄酮含量。

Compositions of leaf surface exudates of different tobacco varieties and changes of cembratrien-diols were studied, sensory quality of flue-cured tobacco leaves was also evaluated in order to provide an effective method of finding new tobacco species resources with special aroma. Surface exudates compositions in 60-day-age and mature stage cutters of 7 tobacco varieties, namely 8306, 12451, Yunyan87, Y892 × 8306, Yuyan No. 11, NX232 and Zhongyan100 were determined with GC-MS method. Expression level of CYC-1 and CYP71D16, solanone content and sensory quality in flue-cured tobacco leaves was analyzed. Results showed that 13 flavor substances in compositions of leaf surface exudates in 60-day-age cutters of the 7 tobacco varieties were detected with cembratriene-diols being the main substance. The content of cembratriene-diols, being the main flavor composition, varied among varieties with the highest content in 8306 at 60 days after leaf sprouting, followed by Yuyan No. 11 and 12451. Expression level of CYC-1 and CYP71D 16 in 8306 was significantly higher than that in 12451 and Yunyan87 while8306 and Yuyan No. 11 had higher content of solanone.