非小细胞肺癌表皮生长因子受体基因突变检测及其与临床病理特征的关系

Non-small Cell Lung Cancer Epidermal Growth Factor Receptor Gene Mu-tation Detection and Its Relationship with the Clinicopathological Character-istics

目的探讨非小细胞肺癌(NSCLC)表皮生长因子受体(EGFR)基因突变情况及其与临床病理的关系。方法对2010年1月—2014年1月来该院诊治的159份非小细胞肺癌手术切除标本进行分析,实验中采用基因测序方法对标本中的EGFR基因突变情况进行检测,分析其与临床病理的关系。结果 159例样本中,EGFR基因突变检出率12.6%(20/159),突变主要集中在19号外显子的缺失和21号外显子的点突变。女性患者基因突变检出率明显高于男性患者(P﹤0.01)。腺癌及细支气管肺泡癌患者基因突变检出率明显高于其他组织学分型(P﹤0.01)。高分化患者基因突变检出率高于中-低分化检出率(P<0.05)。EGFR基因突变与年龄及淋巴结转移与否无关(P>0.05)。结论非小细胞肺癌患者EGFR基因突变与性别、组织学分型及分化程度密切相关。

Objective To investigate the non-small cell lung cancer（NSCLC） epidermal growth factor receptor（EGFR） gene mutation and its relationship with clinical pathology. Methods 159 patients with NSCLC were diagnosed and treated in our hospital from January 2010 to January 2014. And the surgical resection specimens of them were analyzed. Gene sequencing method was used to detect the EGFR gene mutation in the specimens, and the relationship between the EGFR gene mutation and clinical pathology was analyzed. Results In 159 samples, the EGFR gene mutation detection rate was 12.6%（20/159）. The gene mutations mainly were the deletion in exon 19 and exon 21 point mutation. The gene mutation detection rate of the female patients was significantly higher than that of the male patients（P〈0.01）. The gene mutation detection rates of adenocarcinoma and bronchioloalveolar carcinoma were significantly higher than those of other histological types（P〈0.01）. The gene mutation detection rate of patients with high differentiation was higher than that of those with medium or low differentiation（P〈0.05）. EGFR gene mutation had nothing to do with age, and the metastasis of lymph node（P〉0.05）. Conclusion EGFR mutation in patients with non-small cell lung cancer is closely related with the sex, histological type and differentiation degree.