土耳其斯坦叶螨对转基因棉花的寄主选择性

Host Plant Preference of Tetranychus turkestani to Transgenic Cotton

为了研究土耳其斯坦叶螨对转基因棉及其亲本常规棉寄主选择性,以及转基因植物生化特性及形态特征对叶螨寄主选择性的影响,以转基因棉花中棉41、SGK321及其亲本常规棉中棉23、石远321为材料,通过半叶碟选择性试验研究棉叶螨对棉花的取食选择性及产卵选择性,并测定不同品种棉花的营养物质和次生代谢物的质量分数及形态指标。结果表明,棉叶螨对常规棉中棉23和石远321表现出明显的取食偏好和产卵偏好性;转基因棉花中次生代谢物棉酚的质量分数显著高于亲本棉花。因此,土耳其斯坦叶螨对常规亲本棉花的寄主偏好性可能与外源基因导入引起植株代谢变化有一定关系。

In order to study host plant preference of Tetranychusturkestanito transgenic cotton and its isotypic non-transgenic cotton,as well as the relationship between host preference of spider mites with nutrients and secondary metabolites,feeding preference and oviposition preference of cotton spider mites to Zhongmian 41,SGK321 and their corresponding non-transformed isoline Zhongmian 23,Shiyuan 321 were investigated by using dual-choice disc tests.In addition,nutrients,secondary metabolites and morphological characteristic of cotton leaves of different cotton varieties were determined.The results indicated that spider mite showed preference to Zhongmian 23 and Shiyuan 321,which had lower content of gossypol compared with genetically modified cotton.It appeared that genetic transformation changed metabolic pathway,resulting in a higher preference of spider mites to non-transformed cotton varieties.