葡萄柚组织培养快繁体系的建立

Establishment of a rapid propagation system in vitro for Citrus paradisi Macf.

【目的】建立葡萄柚（Citrus paradisi Macf.）稳定高效的离体快繁体系,为葡萄柚遗传转化体系的建立及大规模推广奠定基础。【方法】以引进的亨路比、哈路比、帕利斯、里德马叙和泰国种葡萄柚的无菌苗带芽茎段为外植体,研究不同浓度6-BA、IBA及不同基因型对其丛生芽诱导、增殖及生根培养的影响。【结果】6-BA浓度为0.25和0.50 mg/L时,葡萄柚丛生芽诱导率可达100.0%;IBA浓度为0.10-0.40 mg/L均可诱导出丛生芽。在MS＋0.50 mg/L 6-BA＋0.20mg/L IBA培养基中,帕利斯和泰国种生芽率最高,达96.67%和97.78%。MS＋0.25 mg/L 6-BA＋0.20 mg/L IBA培养基适宜哈路比和里德马叙丛生芽增殖。1/2MS＋2.00 mg/L IBA生根培养基适合哈路比、帕利斯和里德马叙生根,1/2MS＋1.00 mg/L IBA适宜其他葡萄柚品种生根。炼苗移栽成活率以泰国种最高,达87.88%,亨路比最低,仅48.48%。【结论】5个葡萄柚品种腋芽均可进行离体快速培养,并可建立相应的离体快繁体系。

【Objective】A stable and efficient in vitro propagation system for grapefruit was established in order to provide useful references for grapefruit gene transformation and large-scale expansion. 【Method 】The stems with auxiliary buds of aseptic seedlings from introduced Henderson Ruby, Hudson Foster, Perlist, Reed Marsh, and Thailand were used as explants to explore the effects of different concentrations of 6-BA, IBA and different genotypes on the induction and proliferation of adventitious bud, and rooting culture. 【Result 】The highest budding rate of grapefruit was observed in the medium with 0.25 mg/L and 0.50 mg/L 6-BA, which was 100.0%. In the range of 0.10-0.40 mg/L IBA, the adventitious buds were induced. In the medium MS＋0.50 mg/L 6-BA＋0.20 mg/L IBA, Perlist and Thailand had the highest budding rate, which was 96.67% and 97.78%. The medium for multiplication of the regeneration buds of Hudson Foster and Reed Marsh was MS＋0.25 mg/L 6-BA＋0.20 mg/L IBA. The rooting medium of Hudson Foster,Perlist and Reed Marsh was 1/2MS ＋2.00 mg/L IBA. The medium for rooting of the other grapefruit varieties was 1/2MS ＋1.00 mg/L IBA. The survival rate of regenerative plant was the highest in Thailand, which was 87.88 %. The lowest was Henderson Ruby, which was only 48. 48 %. 【 Conclusion 】 The rapid in vitro regeneration system is estab-lished by means of culturing the stems with auxiliary buds from five varieties of grapefruit.