百裕银杏内酯注射液与阿司匹林、氯吡格雷联用对家兔血小板聚集的影响

The effects of bai yu ginkgolide injection combined with aspirin,clopidogrel on platelet aggregation and ultra structure changes

目的观察百裕银杏内酯注射液分别与阿司匹林、氯吡格雷联用后对家兔血小板聚集的影响。方法观察各组对AA、ADP及PAF诱导的家兔血小板聚集作用的影响,以及PAF诱导下血小板超微结构和PF-4和β-TG水平的变化。结果百裕银杏内酯注射液分别与阿司匹林、氯吡格雷联用后,均能抑制PAF诱导的家兔血小板聚集,减少聚集型血小板数量使树突型血小板突起变少变短,并使得PF-4和β-TG表达降低。结论百裕银杏内酯注射液与阿司匹林、氯吡格雷联用后,起到拮抗PAF诱导的血小板聚集的协同作用。

Objective To observe the effects of bai yu ginkgolide injection combined with aspirin, clopidogrel on platelet aggregation in rabbits. Methods In vitro experiments, arachidonic acid （AA）, adenosine diphosphate （ADP） and platelet activating factor （PAF） induced platelet aggregation PAF also induced ultra- structure al- terations. The platelet factor 4 （PF -4） and β -thromboglobulin （β -TG） levels were measured. Results bai yu ginkgolide injection combined with aspirin, clopidogrel could inhibit PAF- induced platelet aggregation, reduce the number of aggregation - type platelets, result in fewer and shorter dendritic platelets after PAF and reduce the levels of PF -4 and β - TG significantly （P 〈 0.0h ）. Conclusion bai yu ginkgolide injection combined with aspi- rin, clopidogrel could produce cooperative effects to prevent PAF- induced platelet aggregation.