自组装短肽RAD16-I在水溶液中对疏水性化合物的稳定作用

Effects of self-assembling peptide RAD16-I on stabilizing hydrophobic compounds in water

目的 研究自组装短肽RAD16-I在水溶液中对疏水性化合物的稳定作用。方法 观察模式疏水性化合物芘在RAD16-I水溶液中于磁力搅拌下形成胶体混悬液的情形,以动态光散射激光粒度仪测定混悬液中粒子的粒度分布,以荧光分光光度法表征芘在与RAD16-I形成的胶体混悬液中的存在形式并考察当此混悬液与卵磷脂（EPC）脂质体混合时,其中芘向模拟细胞的卵磷脂（EPC）脂质体的膜中释放的可能性。结果 在机械搅拌下,芘在RAD16-I水溶液中能形成相对稳定的混悬液,混悬液中粒子粒度为500~5000 nm,平均粒径为1800 nm,芘的荧光发射光谱和激发光谱都表明,芘在此混悬液中以微晶形式存在;当该混悬液与EPC脂质体溶液混合时,芘以分子形式扩散进入EPC脂质体膜中。结论 自组装短肽RAD16-I能在水性体系中稳定疏水性化合物,具有作为疏水性化合物和水难溶性药物载体的潜力。

Objective To investigate effects of self- assembling peptide RAD16 -I on stabilizing hydrophobic compounds in water. Methods Colloidal suspensions of model hydrophobie compound, pyrene, were prepared with the RAD16 -I peptide in water under magnetic stirring; the particle size distribution in the suspension was characterized through dynamic light scattering, and the existence form of pyrene in the colloidal suspensions was characterized and the possibility of pyrene being transferred from the suspensions into membranes of phosphatidyl- choline liposomes was detected through fluorescence spectrophotometry. Results Relatively stable suspension of pyrene, with average diameter about 1800 nm, can be formed with RAD16 -I in water under magnetic stirring and took a form of microcrystal in the suspension, suggested by fluorescence emission and excitation spectra; when the suspension was mixed with phosphatidylcholine liposomes, pyrene microerystals in the suspensions can be molecularly transferred into membranes of the liposomes. Conclusion The self - assembling peptide RAD16 - I can stabilize pyrene in water, thus has the potential as carriers for hydrophobie compounds or drugs.