长链脂肪酸氧化酶在妊娠中期子痫前期样母鼠和胎鼠组织中的表达

Expression of long chain fatty acid oxidase in maternal and fetal tissues in preeclampsia-like mouse model in mid-gestation

目的 探讨长链脂肪酸氧化酶LCHAD在妊娠中期子痫前期样改变母鼠和胎鼠组织中的蛋白表达及分布.方法 将C57BL/6J孕鼠于孕1id皮下注射一氧化氮合酶抑制剂建立子痫前期样模型,同时设立注射生理盐水的正常妊娠对照组,模型鉴定成功后,于孕14 d进行标本取材.采用免疫组化方法检测母鼠及胎鼠组织中LCHAD蛋白的分布和表达状况.结果 孕14 d实验组平均动脉压（115.6±3.3）mmHg（1 mmHg =0.133 kPa）和尿蛋白（97.0±4.3）μg·g体质量^-1·24h^-1较对照组平均动脉压（96.2±4.9）mmHg和尿蛋白（60.8 ±4.9） μg·g体质量^-1·24 h^-1显著升高（P＜0.05）.在两组动物中均可见到母鼠肝、肾、心、胎盘组织中LCHAD蛋白呈阳性表达,实验组母体肝、胎盘LCHAD的IA值强度较对照组明显降低（P＜0.05）.胎鼠的肝、肾、心、肺和神经视网膜组织中,两组均有LCHAD表达,但IA值差异无统计学意义（P＞0.05）.结论 妊娠中期LCHAD蛋白在母鼠和胎鼠重要器官均广泛表达分布,在妊娠期LCHAD与母体能量代谢、胎盘的发育及胎儿生长发育有关;子痫前期样改变时母体肝脏和胎盘的LCHAD表达存在异常.

Objective To explore the protein expression and distribution of long-chain 3-hydroxyacyl-CoA dehydrogenase （LCHAD） in various maternal and fetal tissues in preeclampsia-like and normal pregnant mouse models during the middle trimester.Methods C57BL/6J mice were divided into preeclampsia-like experimental group with Nw-nitro-L-arginine-methyl ester injection and normal pregnancy control group with normal saline injection.Samples were taken at Day 14 of gestation.Immunohistochemistry was used to detect the LCHAD protein expression and distribution in various maternal and fetal tissues.Results The mean arterial pressure was （115.6 ± 3.3） mmHg （1 mmHg =0.133 kPa） in experimental group and （96.2 ± 4.9） mmHg in control group （P ＜ 0.05）.The urine protein contents were （97.0 ± 4.3） μg/g body weight/24h in experimental group and （60.8 ± 4.9） μg/g body weight/24h in control group （P ＜ 0.05）.In both groups,LCHAD protein was expressed in maternal liver,kidney,heart and placenta tissues during the middle trimester.And maternal hepatic tissue,myocardial tissue,renal tubule and spongiotrophoblast showed a strong positive expression,placental labyrinth layer a moderate positive expression and glomerulus a weakly positive expression.LCHAD expression of maternal liver and placenta decreased significantly in experimental group （P ＜0.05）.At Day 14 of pregnancy,LCHAD was expressed in fetal liver,kidney,heart,lung tissue and neural retina.And renal tubule,pulmonary epithelial tissue and neural retina showed a strong positive expression,fetal liver a moderate positive expression and myocardial tissue and glomerulus a weakly positive expression.There was no significant inter-group difference in fetal tissues.Conclusion LCHAD is expressed and distributed widely in maternal and fetal tissues during the middle trimester.And it may play an important role in maternal metabolism and placentafetus development during pregnancy.There is an abnormal protein expression LCHAD in maternal liver and placenta with preeclampsia-like changes.