人龈沟液中MMP-2及TIMP-2在牙移动过程中表达的研究

Study on the functions of MMP-2 and TIMP-2 in human gingival crevicularfluid duringtooth movement

目的观察龈沟液中基质金属蛋白酶-2(MMP-2)和金属蛋白酶组织抑制因子-2(TIMP-2)的含量随作用于人体牙齿正畸力大小的变化,探讨在牙移动过程中,MMP-2和TIMP-2的作用与正畸力的关系。方法收集患者24例并随机分3组:对照组(0 N),轻力组(0.5 N),中度力组(1.5 N)。分别牵引上颌尖牙向远中加力0,7,14,21,28 d,收集龈沟液,应用蛋白质印迹法(Western blot)测量龈沟液中MMP-2与TIMP-2含量的变化。结果实验组中MMP-2、TIMP-2的含量呈波动性变化,并存在有时间依赖性,各组间差异有显著性(P<0.05)。结论轻、中度力下,MMP-2和TIMP-2的表达水平呈显著的波动性变化,并有一定的时间依赖性;MMP-2与TIMP-2参与牙周组织改建过程,二者的动态平衡对牙移动有重要作用。

Objective To observe the changes of the contents of matrix metalloproteinase（ MMP-2） and tissue inhibitor of metalloproteinase（ TIMP-2） in the gingival crevicular fluid（ GCF） varying with the differences of orthodontic traction on human teeth,and to explore the relationships between MMP-2 and TIMP-2 functions and the orthodontic traction in the process of tooth movement. Methods Twenty four patients were selected as subjects and divided into three groups randomly： control group（ 0 N）,mild force group（ 0. 5N）,and moderate force group（ 1. 5 N）. The traction of canine tooth to distal ends was exerted for 0 days,7 days,14 days and 28 days,and then GCF was collected and the changes of MMP-2 and TIMP-2 contents in GCF were measured by western blot. Results In the experimental groups,MMP-2 and TIMP-2 contents have demonstrated volatility changing feature and were time-dependent; there were significant differences between groups（ P〈0. 05）. Conclusions Firstly,the expressions of MMP-2 and TIMP-2 demonstrate volatility changing feature,and are time-dependent under mild and moderate forces. Secondly,MMP-2 and TIMP-2 participate in the process of periodontal tissue remodeling and their dynamic balance plays an important role in tooth movement.