摘要
目的探讨脑胶质瘤共济失调毛细血管扩张突变基因（ATM）及磷脂酰肌醇3-激酶（PI3K）的表达及其意义。方法采用反转录-聚合酶链反应技术检测40例脑胶质瘤组织（WHOⅠ~Ⅱ级25例,WHOⅢ~Ⅳ级15例）和8例非瘤脑组织ATM及PI3K m RNA的表达水平。结果与非瘤脑组织相比,胶质瘤组织ATM和PI3K m RNA水平明显增高（P〈0.05）,而且随着胶质瘤病理级别增高,ATM和PI3K m RNA水平亦明显增高（P〈0.05）。结论脑胶质瘤ATM、PI3K表达显著增高,且随着病理学分级的升高,其表达水平显著升高。这提示两者在胶质瘤发生、发展过程中扮演重要角色。
Objectives To explore the expressions of ataxia-telangiectasia mutated gene （ATM） and phosphatidylinositol 3-kinase （PI3K） in different grades of WHO grading gliomas and their meanings. Methods The expressions of ATM and PI3K mRNA were detected by reverse transcription polymerase chain reaction in 25 samples of WHO grades Ⅰ -Ⅱ, 15 of WHO gradesⅢ -Ⅳ and 8 of normal brain tissues. Results The levels of ATM and PI3K mRNA were significantly higher in WHO grades Ⅲ ~Ⅳ gliomas than those in WHO grades Ⅰ - Ⅱ（P〈0.05）, which were significantly higher than those in the normal brain tissues （P〈0.05）. Conclusions The levels of PI3K and ATM mRNA expressions are significantly higher in the gliomas than those in the normal brain tissues and are positively related to WHO grade of gliomas. It is suggested that the up-regulation of ATM and PI3K expressions may play an important role in the pathogenesis and development of the gliomas.
出处
《中国临床神经外科杂志》
2015年第1期31-33,共3页
Chinese Journal of Clinical Neurosurgery
基金
河南省卫生厅科技创新型人才工程资助项目(201004126)
