摘要
目的:探讨吡非尼酮(Pirfenidone,PFD)对体外培养大鼠角膜基质细胞增殖的抑制效果及其对转化生长因子-β1(transforming growth factor-β1,TGF-β1)表达的影响。方法:分离培养大鼠角膜基质细胞,根据PFD用药的不同浓度分为对照组(0mg/m L)、实验组Ⅰ(0.15mg/m L)、实验组Ⅱ(0.3mg/m L)、实验组Ⅲ(1mg/m L),加药48h后应用CCK-8法检测其对角膜基质细胞增殖能力的影响,免疫细胞化学和Western-blot分别检测ki-67和TGF-β1表达的变化。结果:CCK-8结果显示,相比对照组,各实验组对角膜基质细胞的增殖均有明显的抑制作用,且随浓度的增大其抑制作用明显增强(均P<0.05);免疫细胞化学显示PFD能明显降低ki-67阳性指数(P<0.05);Western-blot结果显示,PFD能降低TGF-β1的表达,且呈剂量依赖关系(P<0.05)。结论:PFD对大鼠角膜基质细胞的增殖具有明显抑制作用,抑制机制与下调TGF-β1表达密切相关,在减轻角膜创伤愈合方面具有潜在的运用前景。
AlM：To investigate the effects of pirfenidone （ PFD） on the proliferation and transfomring growth factor-β1 （ TGF-β1 ） expression in vitro culture rat corneal stromal cells.METHODS： Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL （control group）, 0. 15mg/mL （experimental group▏）, 0. 3mg/mL （experimental group‖）, 1mg/mL （experimental group Ⅲ） for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β1 expression, respectively. RESULTS： Compared with control group, PFD significantly inhibited the proliferation in a dose -dependent manner （ all P 〈0. 05 ）, so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β1 in a dose-dependent manner （P 〈0. 05）.CONCLUSlON： Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β1 expression, therefore, it has potential prospect in lightening the corneal wound healing reaction.
出处
《国际眼科杂志》
CAS
2015年第2期201-204,共4页
International Eye Science
