Ⅲ型前列腺炎病人前列腺液MIF表达及意义

EXPRESSION AND SIGNIFICANCE OF MIF IN TYPE-Ⅲ PROSTATITIS

目的探讨Ⅲ型前列腺炎病人前列腺按摩液(EPS)中巨噬细胞移动抑制因子(MIF)的表达及意义。方法按美国国立卫生院(NIH)前列腺炎诊断标准,用"两杯法"筛选出ⅢA型26例、ⅢB型15例作为研究对象,另选择12例健康男性作为对照组。采用双抗体夹心ELSIA法检测53例受试者EPS中MIF的含量。比较各组之间MIF的表达差异,采用Spearman法分析MIF与慢性前列腺炎疼痛症状评分及EPS中白细胞计数之间的相关性。结果ⅢA组和ⅢB组MIF表达水平均明显高于对照组(F=74.12,q=16.61、13.81,P<0.01),但ⅢA和ⅢB组之间差异无统计学意义(P>0.05)。EPS中MIF水平与慢性前列腺炎疼痛症状评分呈正相关(r=0.48,P<0.01),与白细胞计数无明显相关性。结论 MIF在Ⅲ型前列腺炎病人EPS中的表达增高,且可能与慢性前列腺炎疼痛的发生相关;MIF可能成为诊断Ⅲ型前列腺炎的依据之一。

Objective To explore the expression of macrophage migration-inhibitory factor （MIF） in expressed prostatic secretion （EPS） and its significance in type-m prostatitis. Methods According to the diagnostic criteria of the U.S. National In- stitutes of Health （NIH）, 26 cases of type-Ⅲ and 15 cases of type-Ⅲ B prostatitis were screened using ＂two-cup method＂, and the other 12 healthy men served as normal controls. Using ELISA technique, the content of MIF in EPS in 53 subjects was detec- ted. The correlation between MIF and pain score of chronic prostatitis, and white Mood cell （WBC） count were analyzed by Su- pearman test, Results The levels of MIF in both type-llI A and type-Ill B groups were obviously higher than the control group （F = 74.12;q = 16.61,13.81 P〈0.01）, but the difference of MIF between the type-1]I A and type-IlI B was not statistically signifi- cant （P 〉0.05）, The pain score of chronic prostatitis was positively correlated with the levels of MIF in EPS （r = 0.48, P 〈0.01）, but negatively with WBC count. Conclusion The expression of MIF increases in EPS in patients with type-Ⅲ prostatitis, and is likely to be related with the occurrence of pain of the disease. MIF may become one of the parameters for diagnosing type-Ⅲ prosta titis.