摘要
目的了解雄激素受体阻断剂Flutamide对前列腺癌细胞分化抑制因子1(ID1)表达的影响及意义。方法在培养的前列腺癌LNCaP和PC3细胞中分别加入Flutamide,用实时荧光定量反转录聚合酶链式反应(RTPCR)方法检测ID1mRNA相对定量表达;用Western Blotting方法检测ID1蛋白表达,以不加Flutamide细胞作为对照组。结果与对照组比较,Flutamide组LNCaP和PC3细胞ID1 mRNA相对定量明显减少,LNCaP细胞中ID1蛋白表达明显减少,差异有显著性(t=3.09~34.91,P〈0.05)。Flutamide组LNCaP中ID1 mRNA相对定量、ID1蛋白表达低于PC3细胞,差异有显著性(t=2.57、12.52,P〈0.05)。结论 Flutamide能抑制前列腺癌细胞中ID1的表达,ID1的表达可能受雄激素-受体途径的调控。
Objective To investigate the effect of Flutamide, an androgen-receptor blocker, on the expression of inhibi- tor of differentiation 1 (ID1) in prostate cancer cells. Methods Androgen-dependent and independent prostate cancer cell line- LNCaP and PC3 cells were treated with Flutamide. The relative quantity of ID1 mRNA was detected by SYBR Green I-based real time reverse transcription polymerase chain reaction (RT-PCR). The protein expression of ID1 was detected by Western Blotting. The cells that without Flutamide served as control, group. Results Compared with the control group, the relative quantity of ID1 mRNA in LNCaP and PC3 in Flutamide group significantly decreased (t= 3.09-34,.91, P 〈0.05). The quantity of ID1 mRN A in LNCaP cells was obviously decreased compared with that in PC3 ceils after treated by Flutamide (t = 2.57,12.52 P〈0.05). Con- clusion Flutamide can inhibit the expression of ID1 in prostate cancer cells, suggesting that the expression of ID1 may be regula- ted by androgen-receptor pathway.
出处
《青岛大学医学院学报》
CAS
2015年第1期94-96,共3页
Acta Academiae Medicinae Qingdao Universitatis