三氧化二砷对内皮细胞增殖和一氧化氮分泌的影响

Effect of arsenic trioxide on endothelial cells proliferation and nitric oxide production

目的：通过观察不同浓度三氧化二砷（ As2 O3）对内皮细胞增殖及分泌一氧化氮（ NO）的影响，初步从内皮细胞角度评价As2 O3防治PTCA术后再狭窄的作用。方法以不同浓度As2 O3溶液（0．01、0．1、0．5、1、5、10μmol／L）作用于传代培养的EVC-304细胞，分别培养24、48、72 h测定内皮细胞对3H-TdR摄取量，以硝酸还原酶法测定内皮细胞培养液NO含量。结果 As2 O3浓度低于1μmol／L时对内皮细胞摄取3H-TdR无抑制作用。 As2 O3浓度低于0．1μmol／L时对内皮细胞分泌NO无明显抑制作用；As2 O3浓度0．5～10μmol／L时抑制内皮细胞分泌NO。结论 As2 O3浓度低于0．1μmol／L对内皮细胞增殖及分泌NO均无明显抑制作用；0．1～1μmol／L范围内只对内皮细胞分泌NO的功能有轻度影响，但不抑制内皮细胞的增殖；而较高浓度的As2 O3对内皮细胞增殖及分泌NO均有明显抑制，而且抑制作用随浓度增高而增强。 As2 O3用于防治PTCA术后再狭窄应采取低较浓度。

Objective To investigate the effect of As 2 O3 on proliferation of human umbilical endothelial cells , and nitric oxide （NO）release,and to evaluate the value of NO in inhibiting restenosis .Methods Cultured cells were divided into seven groups , one as control group,the other six groups were incubated with As2O3 at deferent concentrations（0.01,0.1,0.5,1,5,10μmol/L）.Effect of As2O3 on proliferation of human umbilical endothelial cells （ECV-304） was detected by 3H-TdR assay at 24 h,48 h,72 h and nitric ox-ide （ NO） production was elected .Results When the concentration of As 2 O3 was lower than 1μmol/L, it had no effect on endothelial cells proliferation.When the concentration of As 2 O3 was lower than 0.1μmol/L,it had no effect on NO production .NO production was reduced in dose dependent manner ,when endothelial cells were incubated in As 2 O3 within the concentration range of 0.5~10μmol/L. Conclusions When the concentration of As 2 O3 is lower than 0.1 μmol/L,it has no effect on endothelial cells proliferation and NO production.As2 O3 within the concentration range of 0.1~1 μmol/L can reduce NO production , but has no effect on endothelial cells proliferation.As2 O3 at low concentration may be used to reduce restenosis .