摘要
Cancer stem cells have become the new target of chemotherapy with specific cell marker. CD133 is shown to be highly expressed in glioma cancer stem cells. In this study, a drug delivery system is designed to target CD133 by a seven amino acid peptide （TR peptide）, which is capable to specifically bind to CD 133. First, TR was conjugated to DSPE-PEG and coumarin-6 was loaded into the DSPE-PEG micelles. Then fluorescence-activated cell sorting （FACS） and tumorsphere culture were conducted to isolate cancer stern cells in C6 cells. The enhanced uptake of micelles was observed by confocal microscopy and flow cytometry, suggesting that TR peptide-modified micelles may exhibit better anti-cancer efficacy by targeting CD133^＋ glioma stem cells.In conclusion, TR peptide-modified micelles may provide new strategy to achieve enhanced specificity to CD 133^＋ glioma stem cells.
肿瘤干细胞已经成为药物递送的一个新靶点。肿瘤干细胞表面表达特异性标志物,其中CD133在胶质瘤肿瘤干细胞中高表达。因此,我们通过将特异性结合CD133的TR短肽修饰到药物递送系统上,来达到靶向递送药物的胶质瘤干细胞的目的。首先,我们将TR短肽与DSPE-PEG连接,构建包载香豆素-6的主被动DSPE-PEG胶束。之后,我们通过荧光激发免疫细胞分选法和肿瘤球培养法分离胶质瘤肿瘤干细胞。通过共聚焦显微镜和流式细胞仪检测发现,肿瘤干细胞对T R修饰的胶束摄取增多,证明TR修饰胶束具有体外靶向性。由此表明,TR修饰胶束有可能提供了针对CD133+肿瘤干细胞的新的治疗策略。
基金
The Key Program of National Science Foundation(Grant No.81130059)
the National Basic Research Program of China(Grant No.973 program,2013CB932501)
