摘要
Salvia miltiorrhiza （Sm） is a traditional herbal medicine with multiple effects on various diseases. Its water-soluble parts have been used to produce injectional powder. In this study, liver fibrosis rats were induced by intraperitoneal injection of dimethylnitrosarnine for 3 consecutive days per week for 4 weeks. After 2 weeks, rats in the positive drug group were subcutaneously injected with 8×10^5 IU/kg IFNα2b, while the Sm treatment groups were intraperitoneally injected with 50, 100 and 200 mg/kg solution of Sm injectional powder, respectively, for 6 days per week for 4 weeks. The results showed that either IFNα2b or the Sm injectional powder significantly increased the body weight and liver to spleen ratio, and three doses of the powder brought down the spleen index. Serum analysis showed that both IFNα2b and the Sm powder reduced levels of alanine transaminase and total bilirubin, while only 100 and 200 mg/kg of the Sm powder ameliorated aspartate transaminase and albumin levels. In the collagen examination, reduced hyaluronic acid and procollagen type III levels, less fibrous hyperplasia and collagen deposits, and improved hepatocyte states were clearly observed in rats treated with either IFNα2b or Sm injectional powder. In addition, the mechanism of action of the Sm powder was also studied. Immunohistochemical staining showed that IFNα2b and Sm injectional powder significantly down-regulated the expression of transforming growth factor-β1 （TGF-β1） and platelet derived growth factor （PDGF）. In conclusion, Sm injectional powder has protective effects on dimethylnitrosamine-initiated liver fibrosis in rats, and the mechanism may include the down-regulation of TGF-β1 and PDGF.
丹参是一种传统的中草药,对多种疾病有治疗作用,丹参粉针剂主要由其水溶性成分组成。在本项研究中,大鼠腹腔注射二甲基亚硝胺诱导肝纤维化,每周注射3天,连续4周。在造模2周后,阳性药组大鼠皮下注射8×105IU/kg IFNα2b;各治疗组分别腹腔注射50,100和200 mg/kg丹参粉针剂,每周给药6天,连续4周。结果表明,IFNα2b及3个治疗组的大鼠体重和肝脾比明显增加,而且3个治疗组大鼠的脾脏指数降低。血清检测结果显示,IFNα2b和丹参粉针剂可降低谷丙转氨酶,总胆红素,透明质酸,和Ⅲ型前胶原水平,其中100和200 mg/kg剂量组还明显改善了谷草转氨酶、白蛋白水平。在组织学检查中发现,IFNα2b和丹参粉针剂可改善肝细胞的状态,较少的纤维增生和胶原沉积。免疫组织化学染色显示,IFNα2b和丹参粉针剂显著下调了转化生长因子β1和血小板源性生长因子(PDGF)。总之,丹参粉针剂对二甲基亚硝胺导致的大鼠肝纤维化具有一定的保护作用,其机制可能与下调TGF-β1和PDGF有关。
