增殖性糖尿病视网膜病变患者玻璃体液中PEDF与TAOC的关系研究

The expression of pigment epithelium-derived factor and total anti-oxidant capacity in the vitreous fluid of patients with proliferative diabetic retinopathy

目的探讨增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者玻璃体液中色素上皮衍生因子(pigment epithelium-derived factor,PEDF)与总抗氧化能力(total anti-oxidant capacity,TAOC)的关系。方法选择行玻璃体切割术的增殖性糖尿病视网膜病变患者20例(20眼),其中PDR合并玻璃体出血组12眼,PDR无玻璃体出血组8眼。非糖尿病患者18例(18眼)作为对照组。采用ELISA方法测定玻璃体液中PEDF和TAOC的水平,并行统计学分析。结果与对照组比较,PDR患者玻璃体液中的TAOC明显降低(P<0.01)。玻璃体液中PEDF水平与TAOC呈正相关(r=0.35,P<0.05),和对照组呈正相关(r=0.39,P<0.05)。无玻璃体出血组PDR患者玻璃体液中PEDF水平与对照组和合并玻璃体出血组比较明显降低(P<0.05)。结论玻璃体液中PEDF水平与TAOC密切相关。PEDF可作为眼内源性抗氧化物并对PDR起到保护作用。

Objective To observe the expression of pigment epithelium-derived factor （PEDF） and total anti-oxidant capacity （TAOC） in vitreous of patients with proliferative diabetic retinopathy （PDR）, and to investigate the relationship between PEDF and TAOC. Methods Vitreous levels of PEDF and TAOC were measured by an ELISA in 20 eyes of 20 diabetic patients with PDR and in 18 eyes of 18 non-diabetic control subjects. Results TAOC in vitreous was significantly lower in diabetic patients with PDR than that in con- trol subjects （0.18±0.04 mmol/L vs 0.26±0.05 mmol/L, P 〈0.01）. PEDF levels were positively correla- ted with TAOC in the vitreous of patients with PDR （r =0.35 ,P 〈0.05） and control subjects （r =0.39, P 〈0.05）. Further, PEDF levels in vitreous of PDR patients without vitreous hemorrhage were significantly decreased （P 〈0.05）, compared with those in the control or PDR patients with vitreous hemorrhage （4.1 ±2.4 μg/mL, 7.4±3.1 μg/mL, 9.1±4.1 μg/mL）. Conclusion The study demonstrates that PEDF is as- sociated with TAOC in vitreous, suggesting that PEDF may act as an endogenous anti-oxidant in the eye and could play a protective role against PDR.