粒径对多类中药材NIR频谱区的检测研究

Detection analysis of particle size effects in overtone and combination region of NIR based on multi-types of Chinese material medica

采用近红外(NIR)光谱漫反射分析技术,提出基于频谱特征,分析粉末粒径对多类中药材近红外漫反射光谱检测影响。以多类中药材为载体,将其粉碎过60,80,100,120目筛,采集近红外光谱。以柴葛根、柴胡、山药为载体考察中药材装样过程所引入误差。结果表明近红外光谱不同波段受粒径的影响不同,在组合频区和一级倍频区的近红外光谱强度与粒径成正比,而且随着波长增大,受粒径的影响越大。而在二级倍频区,近红外光谱强度与粒径成正比。对于装样误差的考察结果表明,当粉碎目数大于60目时,装样误差较小。阐明了合适的粉碎粒径,保证中药近红外漫反射光谱分析技术的准确性。

Near-infrared （NIR） diffuse reflectance spectroscopy was used to analyze the impact of multi-class particle size of Chi- nese material medica（CMM） based on the spectral characteristics in overtone and combination region. Several types of CMM（60, 80, 100,120 mesh） were subjected to NIR spectra analysis. Spectral reproducibility was examined after sample repackage. The result showed that the effects of particle size on the NIR spectra were different according to different bands, in the combination region and first combination-overtone region. Spectroscopy intensity was proportional to the particle size and influence of particle size was greater as the wavelength increased. While in the second combination-overtone region, it was inversely proportional to particle size. To the sampling loading error, the resuh indicated that when the mesh number was larger than 60 mesh, the error was small. The appropriate particle size was clarified to guarantee the accuracy and reliability of NIR diffuse reflectance spectroscopy in CMM.