藏药沙棘膏HPLC指纹图谱研究

HPLC fingerprint of Tibetan medicine Shaji Gao

通过对不同产地藏药沙棘膏的HPLC指纹图谱分析,为沙棘膏的品种鉴定和制备工艺的研究奠定基础。实验使用Agilent zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.4%磷酸溶液为流动相梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长360 nm。建立了15个批次沙棘膏的共有图谱,提取了7个色谱峰作为指纹图谱共有峰,结合对照品对其中的3个色谱峰进行了指认,分别为槲皮素、山柰素、异鼠李素;15批药材中第14批次与对照指纹图谱比较相似度小于0.9,其他批次的相似度均大于0.9。该研究首次采用HPLC建立藏药沙棘膏的指纹图谱,该方法具有良好的精密度、稳定性和重复性,为沙棘膏质量控制和评价提供依据。

This study established an HPLC fingerprint of Tibetan medicine Shaji Gao from different habitats and lay a foundation for Shaji Gao varieties identification and preparation process. The chromatographic condition was as follow： Agilent zorbax SB-C18 （4. 6 mm ×250 mm, 5 μm）eluted with the mobile phases of acetonitrile and 0. 4% phosphoric acid water in gradient mode. The flow rate was 1.0 m L·min^-1 , and the detection wavelength was set at 360 nm. The fingerprints of 15 batches Shaji Gao were carried out by similarity comparation, 7 chromatographic peaks were extracted as the common peaks of fingerprint, 3 peaks were identified, which were quercetin, kaempferol and isorhamnetin. The similarity degrees of 14 batches of samples were above O. 9 and 1 batch of samples was below 0. 9. This is the first established fingerprint of Shaji Gao by using HPLC. This method has good precision, stability and re- peatability that it could provide basis for quality control and evaluation of Shaji Gao.