新塔花药材中3种指标性成分随生长周期的变化规律

Study on Change Regulations of 3 Indicative Components in Ziziphora bungeana during the Growth Cycle

目的:探讨新塔花中胡薄荷酮、咖啡酸与迷迭香酸在不同生长周期中的积累量及其变化规律。方法:采集处于不同生长周期的新塔花药材,采用高效液相色谱法进行含量测定。色谱柱为Agilent-C18,流动相为甲醇-0.3%磷酸水溶液(梯度洗脱),检测波长为290 nm,柱温为30℃,流速为1.0 ml/min,进样量为10μl。结果:从4月底返青至10月初种子成熟的整个生长周期,3种指标性成分含量均呈现出先升高后降低的趋势,胡薄荷酮与咖啡酸于7月中旬至8月中旬在药材中的含量较高,迷迭香酸于6月下旬至8月初在药材中的含量较高,整体药材于7月中旬至8月初的质量最好。结论:新塔花药材中指标性成分的含量变化随生长周期表现出较好的规律性,7月中旬至8月初的新塔花药材质量最好。这可为新塔花药材的采收与生产加工提供依据。

OBJECTIVE： To explore the accumulative amount and change regulations of pulegone, caffeic acid and rosmarinic acid in Ziziphora bungeana during different growth cycles. METHODS： Z. bungeana were collected during different growth periods and HPLC was adopted for the content determination. The determination was performed on Agilent-C18 column with mobile phase consisted of methanol-0.3% phosphoric acid （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set at 290 nm, and the column temperature was 30 ℃. The sample size was 10 μl. RESULTS： The three indicative components increased at first after reviving at the end of April, but later decreased when seeds matured at the early October; the contents of pulegone and caffeic acid were higher from mid-July to mid-August; and the contents of rosmarinic acid was higher in the late June to early Au- gust. So the quality of Z. bungeana during mid-July and early August was the best. CONCLUSIONS： The content changes of indica- tive components of Z. bungeana show good regularity with growth cycle. The quality of Z. bungeana during mid-July and early Au- gust is the best. It lays the foundations for the harvest, processing and production of Z. bungeana.