白茅根配方颗粒的HPLC特征图谱研究

Study on HPLC Characteristic Chromatogram of Imperata cylindrica Granules

目的:建立白茅根配方颗粒的高效液相色谱(HPLC)特征图谱。方法:采用HPLC法。色谱柱为Megres5-C18,流动相为乙腈-0.05%磷酸水溶液(梯度洗脱),流速为1.0 ml/min,柱温为30℃,检测波长为327 nm,进样量为10μl。以绿原酸峰为参照物,分析12批白茅根配方颗粒样品,采用《中药色谱指纹图谱相似度评价系统》进行相似度评价。结果:白茅根配方颗粒的特征图谱由5个共有峰构成,12批样品的相似度≥0.926。经验证,白茅根配方颗粒中的特征峰与白茅根饮片水煎液中的色谱峰能够一一对应。结论:所建立的特征图谱可为白茅根配方颗粒的鉴别和质量评价提供参考。

OBJECTIVE： To establish the HPLC characteristic chromatogram of Imperata cylindrica granule. METHODS： HPLC method was adopted. The determination was performed on Megres5-C18 column with mobile phase consisted of acetoni- trile-0.05% phosphate solution （gradient elution） at the flow rate of 1.0 ml/min. The column temperature was 30 ℃, and detection wavelength was set at and 327 nm. The sample size was 10 μl. Using the peak of chlorogenic acid as control, 12 batches of/. cylin- drica granules were analyzed; TCM chromatogram fingerprint similarity evaluation system was adopted to evaluate similarity. RE- SULTS： There were 5 common peaks in the characteristic chromatogram of 1. cylindrica granules, and the similarity of 12 batches of sample was higher than 0.926. There was one-to-one match between characteristic peaks of 1. cylindrica granules and chromato- gram peaks of L cylindrical decoction pieces. CONCLUSIONS： The established characteristic chromatogram can provide reference for the quality control and identification of 1. cylindrica granules.