Ki-67、P16、CK17在鉴别宫颈非典型鳞化、未成熟鳞化中的意义

Significance of Ki-67,P16,CK17 in discriminate of atypical immature metaplasia and immature squamous metaplasia

目的研究Ki-67、P16、CK17在宫颈非典型鳞化(AIM)、未成熟鳞化(IM)中的表达,以探讨Ki-67、P16、CK17对于AIM与IM的鉴别诊断意义。方法应用免疫组化技术检测诊断为AIM及IM的100例患者中Ki-67、P16及CK17的表达情况,并进行统计学分析。结果 100例患者中57例AIM,43例IM,与原诊断比较误诊率达12%。57例AIM Ki-67阳性率75.4%,P16阳性率77.2%,CK17阳性率35.1%;43例IM中Ki-67阳性率4.7%,P16阳性率16.3%,CK17阳性率97.7%,差异均有统计学意义(P<0.01)。Ki-67、P16、CK17联合检测方法均优于Ki-67、P16联合检测和P16、CK17联合检测,差异均有统计学意义(P<0.05)。结论 Ki-67、P16、CK17联合检测对于AIM与IM的鉴别诊断有重要意义。

Objective To study the expression of Ki-67,P16,CK17 in cervical atypical immature metaplasia（AIM）,immature metaplasia（IM） in order to explore differential diagnosis significance of Ki-67,P16,CK17 in AIM and IM. Methods Immunohistochemical staining was used to detect the expression of Ki-67,P16 and CK17 in patients with AIM and IM（n=100）,and the results were statistically analyzed. Results In 100 patients,57 cases of AIM,43 cases of IM,compared with the original diagnosis,misdiagnosis rate was 12%.In 57 cases of AIM,the positive rate of Ki-67 was 75.4%,the positive rate of P16 was 77.2%,the positive rate of CK17 was 35.1%;In 43 cases of IM,the positive rate of Ki-67 was4.7%,the positive rate of P16 was 16.3%,the positive rate of CK17 was 97.7%,with statistical difference（P〈0.01）.The combined detection method of Ki-67,P16,CK17 was better than that combined detection of Ki-67,P16 and combined detection of P16,CK17 respectively,with statistical difference（P〈0.05）. Conclusion The combined detection of Ki-67,P16,CK17 has important significance for the differential diagnosis of AIM and IM.