碱蓬DNA导入玉米变异后代耐盐性研究及SSR分析

SSR Analysis and Study on the Salt-tolerance of Descendant Variation Induced by Introducing Suaeda Salsa DNA into the Maize

研究导入野生碱蓬DNA的玉米自交系后代的耐盐性及分子水平的变异,从中筛选出较强的耐盐新材料,为玉米耐盐育种提供种质资源。结果表明,在250 mmol/L的Na Cl胁迫后,导入系K-3与其他导入系及受体相比存活绿叶多,鲜重和干重变化率较低,耐盐性明显。SSR分析得出,引物phi065标记座位检测到导入系K-3与供体有相同的带型,phi077标记座位检测到导入系K-3还产生与受体和供体均不相同的带型,分析导入系K-3的耐盐性可能是由于整合了部分野生碱蓬DNA片段的结果所致。

The maize inbred lines offspring which were induced by introducing suaeda salsa DNA into the maize were used to research the variation of salt resistance and molecular level. The results showed that compared with other material, that introgression lines K-3 had more green leaves, fresh weight and dry weight rate were lower under the 250 mmol/L NaCI stress. This indicated that the introgression lines K-3 had strong salt tolerance. The variation of molecular level analysis was performed by SSR technique. The results found that the same band type was deteeted in the introgression lines K-3 and the donor by the phi 065 markers, and a new band type which was different from donor and receptor was detected by the phi 077 markers. These founds implied that the salt tolerance of K-3 might be integrated into part of suaeda salsa DNA.