蜜柑草三氯甲烷部位化学成分分析

Chemical Constituents of CHCl_3 Extract from Phyllanthus matsumurae

目的：研究广西常用民间草药蜜柑草三氯甲烷萃取部位的化学成分，从中寻找有重要生物活性及药用前景的天然化合物，为其深度开发利用提供科学依据。方法：利用硅胶柱色谱结合凝胶柱色谱等多种分离技术，从壮族药蜜柑学75％乙醇提取物的三氯甲烷萃取部位分离得到7个化合物，并通过理化性质和^1H-NMR，^13C-NMR，MS等波谱学技术进行结构鉴定。结果：从壮族药蜜柑草三氯甲烷萃取部位共分离得到的7个化合物，分别鉴定为槲皮素（1），3，3′-O-二甲基槲皮索（2），3′，4′-O-二甲基槲皮素（3），（-）-表没食子儿茶素（4），3，3′-O-二甲基鞣花酸（5），3，4-O-二甲基鞣花酸（6），β-胡萝卜苷（7）。结论：化合物2-7为首次从该植物中分离得到。

Objective： To study chemical constituents of CHCl3 extract from PhyUanthus matsumurae and provide the scientific evidence for further development. Method： Various techniques such as silica gel column and Sephadex LH-20 chromatography were used for the isolation and purification. Physicochemical properties and spectroscopic methods including^1H-NMR,^13C-NMR and MS were used for the identification of structures. Result： Seven compounds were isolated from the CHCl3 extract of 75% alcohol extract of the whole plant and identified to be quercetin （1）, 3, 3′-dimethylquercetin （2）, 3′, 4′-dimethylquercetin （3）, （-）-epigallocatechin （4）, 3, 3′-O-dimethylellagic acid （ 5 ）, 3, 4-O-dimethylellagic acid （ 6 ）, and β-daucosterol （ 7 ）. Conclusion：Compounds 2-7 were reported from this plant for the first time.