HPLC同时测定芪白颗粒中5种活性成分的含量

HPLC Simultaneous Determination of Five Active Constituents in Qibai Granules

目的：建立HPLC同时测定芪白颗粒中5种成分（黄芩苷、人参皂苷Rg1、人参皂苷Rb1、三七皂甘R1和特女贞苷）含量的方法。方法：Prevail C18色谱柱（4.6mm×250mm，5μm），流动相甲醇（A）-0.2％磷酸水溶液（B）梯度洗脱（0-5min，45％A；5-15min，45％-50％A；15-60min，50％A），柱温室温，流速1.0mL·min^-1，检测波长203nm。结果：黄芩苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1和特女贞苷分别在2.16-21.6，1.95-19.5，2.64-26.4，3.51-35.1和1.01-10.1mg·L^-1线性关系良好（r〉0.999），精密度、重复性、稳定性的RSD均〈2％，平均加样回收率95.92％-101.90％。结论：该方法简便、合理、可靠，可作为芪白颗粒质量控制的方法。

Objective： To establish an HPLC method for simultaneous determination of baicalin,ginsenosides Rgl, ginsenoside Rb1, notoginsenoside R1 and special privet glycosides five in Qibai granules. Method： HPLC analysis was performed on a Prevail C18 column （4.6 mm×250mm,5μm） with methanol （A） - 0.2% phosphoric acid （B） as mobile phase by gradient elution [0-5min, 45% A, 5-15 min, 45%-50% A, 15-60min, 50% A].The flow rate was 1.0 mL·min^-1 , column temperature was maintained at room temperature, and the detection wavelength was set at 203 nm. Result： The linear ranges of baicalin, ginsenosides Rgt, ginsenoside Rb1 , notoginsenoside and special privet glycosides were 2.16-21.6, 1.95-19.5, 2.64-26.4, 3.51-35.1 and 1.01-10.1 mg·L^-1, respectively. The RSD of precision, reproducibility and stability tests were less than 2% , the average recoveries were ranging from 95.92% to 101.90%. Conclusion： This method is simple, reasonable,reliable and could be used for quality control of Qibai granules.