UPLC-Q-TOF检测止咳平喘类中成药中非法添加的8种化学药品

Determination of 8 Kinds of Chemical Medicines Illegally Added in Traditional Chinese Medicines for Relieving Cough and Asthma by UPLC-Q-TOF

目的：建立止咳平喘类中成药中非法添加的8种化学药品的检测方法。方法：采用超高效液相色谱-四级杆-琶行时间质谱（UPLC-Q-TOF），MS模式进行检测。采用Waters Acquity BEH C18色谱柱（2.1mm×100mm，1.7μm），流动相甲醇-0.1％甲酸的10mmol·L^-1甲酸铵溶液梯度洗脱，流速0.4mL·min^-1；离子源ESI源，正离子检测，扫描范同m／z 100-600。对茶碱、磺胺甲噁唑、氯苯那敏、苯海拉明、喷托维林、苯丙哌林、醋酸泼尼松、地西泮进行定量俭测。结果：8种化学药品的线性范围分别为1.251-62.55，1.043-52.15，1.057-52.85，1.002-50.10，1.147-57.35，1.069-53.45，0.991-49.55，1.111-55.55mg·L^-1，8种化学药品平均回收率和97.8％-103.2％。结论：该方法快速、简便、准确，可用于止咳平喘类中成药中非法添加8种化学药品的快速检测。

Objective： To establish an UPLC-Q-TOF method for the determination of 8 kinds of chemical medicines illegally added in traditional Chinese medicines for relieving cough and asthma. Method： UPLC-Q-TOF and MS mode was adopted to perform the quantitative determination with the Waters Acquity BEH C18 column （2.1 mm×100mm,1.7μm）, methanol -10 mmol·L^-1 ammonium formate （containing 0.1% formie acid） for the gradient elution, and the flow rate was 0.4 mL·min^-1 The ion source was eleetrospray ionization （ESI） with the positive ion detection. The scan range was m/z 100-600. Theophylline, sulfamethoxazole, chlorphenamine, diphenhydramine, pentoxyverine, benproperine, prednisone acetate and diazepam added in the traditional Chinese medicines were quantitatively identified. Result： The linear ranges of 8 kinds of chemical medicines were 1.251-62.55, 1.043-52.15, 1.057-52.85, 1.002-50.10, 1.147-57.35, 1.069-53.45, 0.991-49.55, 1.111-55.55 mg·L^-1 The average recovery rate of 8 kinds of chemical medicines was 97.8%-103.2%. Conclusion ： This method is rapid, simple and accurate, and could be used to detect 8 kinds of chemical medicines added in traditional Chinese medicines for relieving cough and asthma.