红芸豆α-淀粉酶抑制剂与中药组合饲料的减肥降脂降糖作用

Effect on Weight Loss,Glucose and Lipid-lowering of Food Containing Combination of α-amylase Inhibitor from Red Kidney Bean and Traditional Chinese Herbal Medicines

目的:进行安全而有效的减肥降脂食品研究具有广泛的应用价值,本文探讨了红芸豆α-淀粉酶抑制剂、决明子、山楂以及丹参等不同组合配方对小鼠的减肥降脂和降糖作用。方法:昆明种小鼠44只分为正常组、饲料1组、饲料2组、饲料3组,采用小鼠饲喂配合了芸豆α-淀粉酶抑制剂、决明子、山楂、丹参等植物药成分的高脂饲料,饲养30 d后,观察各组小鼠的体重变化及对脏器系数的影响,采用全自动生化分析仪进行肝功能丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),白球比,总蛋白(TP)的检测,试剂盒检测小鼠血糖(GLU)及血脂含量。结果:与正常组比较,饲料1组、饲料2组、饲料3组小鼠的体重有所降低,各脏器系数有升高,同样也有降低(P<0.05,P<0.01),各饲料组肝功能ALT,AST及TP含量明显降低,白球比明显升高,各饲料组GLU明显降低,各饲料组小鼠血脂高密度脂蛋白(HDL)含量明显升高,总胆固醇(CHOL)、甘油三酯(TG)含量明显降低,低密度脂蛋白(LDL)含量饲料2号明显升高、饲料1号和3号明显降低,均具有一定的差异(P<0.05,P<0.01)。结论:红芸豆α-淀粉酶抑制剂,决明子和山楂三者搭配具有良好的减肥降脂和降糖效果。

Objective： To study the effect of different combinations of herbal medicines and foods on weight loss, glucose and lipid-lowering, this is beneficial to humans and would have a broad application value. Method： Fouty-four Kunming mice were divided into the control group and different combination groups [containing different concents of a-amylase inhibitor extracted from red kidney bean, Cassiae Semen, Cratagei Fructus （Crataegus） and Radix et Rhizoma]. The body weight and organ coefficient were evaluated, the lipid, total cholesterol, and alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, albumin/globulin, total protein （TP）, blood sugarg （glucose, GLU） and blood fat were tested after 30-day treatment. Result： Compared with the normal group, the body weight declined, organ coefficient changed （P 〈 0.05, P 〈 0.01 ） , the ALT, AST arid TP contents reduced, albumin/globulin values raised, GLU level reduced, high-density lipoprotein raised, total cholesterol and triglyceride declined in all combination groups （ P 〈 0.05, P 〈 0.01 ）. Different effects on low density lipoprotein were obtained from the different combination groups （P 〈0.05, P 〈 0.01 ）. Conclusion： The experiment results showed that the a-amylase inhibitor played a significant role in reducing body weight, glucose and lipid-lowering when combined with cassia seed, hawthorn and red sage root.