IPK在肾脏功能分子研究中的应用进展

Application of IPK in Renal Functional Molecules Research

离体肾脏灌流(IPK)是应用比较早的离体器官灌流实验方法,具有去除神经和体液等影响、便于施加干扰因素以及实时监测肾脏功能变化的特点,可用于研究药物肾脏代谢与排泄及其机制等。本文通过对国外利用IPK技术进行研究的文献进行综述,旨在拓展国内利用IPK技术进行研究的视角。通过整理、归纳、分析大量国外利用IPK技术进行研究的文献,本文主要从肾小球滤过调节机制、肾小管和集合管重吸收和分泌调节机制以及肾脏局部缺血损伤模型等多个方面探讨IPK技术在研究肾脏分子水平上的变化以及肾脏发病机制和肾病发生发展过程中各调节因素相互作用等方面的应用。通过大量文献的分析,发现国外运用IPK技术比较成熟,而国内运用此技术主要研究药物的代谢排泄机制、药物性肾损伤机制或供体肾脏移植等方面,较为局限。本文针对IPK技术在肾脏分子水平上的变化以及肾脏发病机制等研究中的应用进行介绍,可以为国内学者利用IPK技术在研究药物以及致病因素刺激下肾脏功能改变及其机制等方面提供参考,并指出IPK技术在整体肾脏角度研究足细胞形态结构和功能改变及其分子机制方面有重要意义。

As an early isolated organ perfusion method, the isolated perfused kidney （IPK） has many advantages, such as the removal of nerves and humoral factors, convenience to exert interference factors and real- time monitoring the diversity of renal function. The IPK has great application value in studying drug metabolism, excretion and revelant mechanisms in kidney. This article aims to expand the domestic perspective of using IPK. This paper discussed the applications of IPK in the study of changes in kidney molecules, pathogenesis and interaction of various adjustment factors in nephropathy development process from many aspects, such as regulatory mechanisms of glomerular filtration, reabsorption and secretion of tubule and collecting duct, and renal ischemia injary model. By analyzing a large number of documents, we found that the using of IPK in foreign is relative!y mature, while the domestic is more limited, mainly applied in metabolic excretion mechanism of drugs, mechanisms of drug-induced renal injury or donor kidney transplants, etc. This article described the use of IPK technology in the research of the molecular level changes in the kidney and renal mechanisms of pathogenesis. It could provide a reference for the domestic study of changes in renal function and mechanism stimulated by me Jication and risk factors. It also noted that IPK technology would be very important in morphological and functional changes of podocytes and its molecular mechanisms in the perspective of the whole kidney.