摘要
以成熟期黑皮果蔗(Badila)的第2、5、8、12节间为材料,分析测定其节间蔗糖质量分数与相关关键酶如蔗糖磷酸合成酶(sucrose-phosphate synthase,SPS)、可溶性酸性转化酶(soluble acid invertase,SAI)、中性转化酶(neutral invertase,NI)活性,并采用RT-PCR对SPS、SAI基因在4个不同节间部位中的表达差异进行半定量分析。结果表明,成熟期黑皮果蔗蔗茎中不同节间的蔗糖、还原糖质量分数均随着节间的成熟而增加,成熟节间>未成熟节间;蛋白质质量分数则相反,为未成熟节间高于成熟节间。SPS活性与SPSⅢ基因表达则是在幼嫩茎的活性高于成熟茎;SAI活性也是幼嫩茎大于成熟茎,其基因表达差异表现不明显。NI活性以第5节间最低,其他3个节间无极显著差异。
The 2nd,5th,8th and 12 th internode of chewing cane(Badila)was used as experimental material to assay the sucrose content and the activity of the key enzymes,i.e.,sucrose-phosphate synthase(SPS),soluble acid invertase(SAI)and neutral invertase(NI)related to sucrose metabolism,and the expressions of genes was also assayed by semi-quantitative RT-PCR at mature stage.The results showed that the contents of sucrose and hexose sugars in different internodes increased with the numbers of internodes,and mature internodes〉 immature internodes;the soluble protein content was contrary and immature internodes 〉mature internodes.The activity of SPS enzyme and the expression of SPSⅢ gene in immature internodes were higher than of mature internodes;the activity of SAI enzyme as the same,but the gene expression had no difference.The activity of NI in No.5was lowest,and there had no difference for other 3internodes.
出处
《西北农业学报》
CAS
CSCD
北大核心
2015年第1期119-122,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
"十二五"农村领域国家科技计划(2013AA102604-1)
广西自然科学基金项目:创新研究团队项目(2011GXNSFF018002)
面上项目(2011GXNSFA018111)
广西科学研究与技术开发计划项目:重点实验室建设项目(12-071-09)
科技攻关计划(桂科攻1222009-1B)
广西农业科学院基本科研业务专项(桂农科2012YT04
200802Z(基))
科技发展基金(2007024)
关键词
黑皮果蔗
蔗糖质量分数
蔗糖代谢相关酶
Chewing cane(Badila)
Mass fraction of sucrose
Activity of enzymes