黑皮果蔗(Badila)节间蔗糖质量分数及其关键酶活性和相关基因表达的分析

Mass Fraction of Sucrose and Activity of Key Enzymes and Related Genes Expression in Internodes of Chewing Cane(Badila)

以成熟期黑皮果蔗(Badila)的第2、5、8、12节间为材料,分析测定其节间蔗糖质量分数与相关关键酶如蔗糖磷酸合成酶(sucrose-phosphate synthase,SPS)、可溶性酸性转化酶(soluble acid invertase,SAI)、中性转化酶(neutral invertase,NI)活性,并采用RT-PCR对SPS、SAI基因在4个不同节间部位中的表达差异进行半定量分析。结果表明,成熟期黑皮果蔗蔗茎中不同节间的蔗糖、还原糖质量分数均随着节间的成熟而增加,成熟节间>未成熟节间;蛋白质质量分数则相反,为未成熟节间高于成熟节间。SPS活性与SPSⅢ基因表达则是在幼嫩茎的活性高于成熟茎;SAI活性也是幼嫩茎大于成熟茎,其基因表达差异表现不明显。NI活性以第5节间最低,其他3个节间无极显著差异。

The 2nd,5th,8th and 12 th internode of chewing cane（Badila）was used as experimental material to assay the sucrose content and the activity of the key enzymes,i.e.,sucrose-phosphate synthase（SPS）,soluble acid invertase（SAI）and neutral invertase（NI）related to sucrose metabolism,and the expressions of genes was also assayed by semi-quantitative RT-PCR at mature stage.The results showed that the contents of sucrose and hexose sugars in different internodes increased with the numbers of internodes,and mature internodes〉 immature internodes;the soluble protein content was contrary and immature internodes 〉mature internodes.The activity of SPS enzyme and the expression of SPSⅢ gene in immature internodes were higher than of mature internodes;the activity of SAI enzyme as the same,but the gene expression had no difference.The activity of NI in No.5was lowest,and there had no difference for other 3internodes.