响应曲面法优化玉米须多糖提取工艺的研究

Study on Extraction Process of Polysaccharide of Corn Silk Optimized by Response Surface Methodology

采用微波辅助,乙醇沉淀法提取玉米须中的多糖,在单因素实验基础上,利用BoxBehnken实验设计原理,设计3因素3水平实验,采用Design-Expert进行响应面分析,建立玉米须多糖提取的回归方程。经检验该回归方程能够较好的预测玉米须多糖的提取率。经响应面优化法确定的最佳工艺条件为:料液比1∶8(g/m L),提取温度93℃、提取时间30 min。经过实验验证,玉米须多糖的提取率为1.13%,与预测值的相对误差为0.88%。

This research makes the polysaccharide extraction of corn silk by means of microwave assisted extraction and the ethanol precipitation method. Based on the single factor experiment results, and according to the experimental design principle of Box -Behnken Center,it designs the experiment of three fac- tors three levels. The software of Design - Expert is adopted to make response surface analysis, thus to build the regression equation of the polysaccharide extraction of corn silk. By checking, this regression equation is reasonable and reliable. It can better predict the extraction rate of the Polysaecharide extraction of corn silk. The optimum condition is determined by the method of response surface for the material liquid ratio 1 ： 8 （ g/ mL）. The extraction temperature is 93℃. The extraction time is 3 min. It is verified by experiment that the extraction rate of Polysaceharide extraction of corn silk is 1.13%, and the relative error with prediction is 0. 88%.