雌激素α受体PvuⅡ和XbaⅠ基因多态性与产后抑郁障碍的相关性

The polymorphisms of Pvu Ⅱ and Xba Ⅰ genes of the estrogen receptor in women with postpartum depression disorder

目的探讨雌激素α受体(ERα)基因多态性与产后抑郁障碍发生的关联性,为产后抑郁障碍发生的病因机制提供遗传学证据,进而为疾病基因诊断与治疗提供理论基础。方法选取产后抑郁障碍产妇(产后抑郁组)与健康产妇(正常对照组)各45例,运用限制性片段长度多态性聚合酶链式反应分析法、基因记数法统计两组ERα基因的基因型和等位基因频率,分析ERαPvuⅡ和XbaⅠ多态性与产后抑郁的关联性。结果产后抑郁组与正常对照组ERαPvuⅡ基因型及等位基因(P与p)频率分布差异有统计学意义(P<0.05),产后抑郁组与正常对照组ERαXbaⅠ基因型及等位基因(X与x)频率分布差异无统计学意义(P>0.05),ERαPvuⅡ与XbaⅠ联合基因型分布差异无统计学意义(P>0.05)。结论 ERαPvuⅡ基因多态性与产后抑郁障碍发病有相关性,XbaⅠ基因型突变对于产后抑郁障碍发病无相关性,联合基因分析,产后抑郁产妇与健康产妇整体基因型分布相似。

Objective To investigate the correlation between the polymorphisms of estrogen receptor- α（ ERα）and postpartum depression disorder（ PPD）,to provide genetic evidences for the etiology and pathogenesis of PPD,ant to provide the theory basis for diagnosis and therapy for PPD. Methods 45 cases（ depressed group） and 45 controls（ control group） were selected according questionnaires. PCR- RFLP was applied to examine the polymorphisms of PvuⅡ and XbaⅠ in ERα. Allele frequency was assessed using genetic numeration. The correlations between the polymorphisms of PvuⅡ and XbaⅠ and PPD using SPSS 18. 0. Results The chi- square test revealed that the genotype distributions of PvuⅡ in ERα in depressed group and control group were significantly different,so were the allele frequencies（ P and p,P〈0. 05）. There was no significant different in genotype distribution or allele frequency（ X and x） of XbaⅠ in ERα between depressed group and control group（ P〉0. 05）. Conclusion There is correlation between polymorphism of ERαPvuⅡ and PPD,while no correlation between genetic mutation of XbaⅠ and PPD is revealed. The analysis of the joint genotype shows that the general genotype distributions in depressed group and control group are similar.