龙葵毛状根诱导条件的研究

Study on Inducing Hairy Roots of Solanum nigrum L.

以发根农杆菌A4、C58C1和A1476为诱导菌株,探讨外植体类型、发根农杆菌种类、侵染时间、预培养时间、菌液浓度和共培养天数对龙葵毛状根诱导率的影响。结果表明:外植体以叶片诱导率最高;发根农杆菌A4、C58C1和A1476侵染龙葵叶片均能诱导出毛状根,C58C1诱导率较高;较佳侵染时间为5min;发根农杆菌浓度在OD600=0.6时诱导效果较好;预培养和共培养时间均为2d时诱导率较高。该试验为大量生产毛状根,并利用毛状根培养技术生产药物龙葵碱提供了试验基础。

Agrobacterium rhizogenes strains of A4,C58C1,A1476 were selected to discuss effect of explants types,Agrobacterium rhizogenes species,infection time,pre-culture time,concentration of bacterium,co-cultivation period on hairy roots inductivity of Solanum nigrum.The results showed that with leaf as explants,the inductivity was the highest.Agrobacterium rhizogenes strains of A4,C58C1,A1476 could all induce hairy roots,but inductivity of C58C1 was higher.Better infection time was 5minutes.The inductivity at OD600=0.6was better performing.The inductivity was higher when the time of pre-culture and co-cultivation was 2days.It provided experimental basis for the mass production of hairy roots and alkaloid of Solanum nigrumproduction which used hairy root culture techniques.