从土茯苓中提取总黄酮的方法研究

Study of Methods on the Extraction of Total Flavonoids from Smilax Glabra Roxb

本文以中药土茯苓为原料,乙醇为提取剂提取总黄酮。探究了普通回流法、浸渍法、索氏提取法对总黄酮粗提物质量的影响,确定最佳提取方法为普通回流法。在普通回流法提取的条件下,进行单因素实验。探究反应时间、反应温度、料液比、浸泡时间、乙醇浓度对总黄酮粗提物质量的影响。得出最佳工艺条件为:反应时间3 h,反应温度80℃,料液比1∶20,乙醇浓度50%,浸泡时间12 h。利用UV对总黄酮粗提物进行定性检测。

The extraction of total flavonoids from Smilax glabra Roxb with ethanol as extracting agent was studied in this article. Effect on the mass of total flavonoids exerted by the three different extraction methods including ordinary reflux method, dipping method and Soxhletg extraction method was explored. Then the ordinary reflux method was determined as the optimal extraction method. Single factor experiments were conducted in the condition of ordinary reflux method. Reaction time, reaction temperature, material - solution ratio, ethanol concentration and soak time were explored. Through series of experiments, the optimal reaction conditions were given. The reaction time was 3h, the reaction temperature was 80℃, the material- solution ratio was 1：20, the ethanol concentration was 50% and the soak time was 12 h. Finally the total fiavonoids can be qualitatively detected by UV.