枸杞多糖对雏鸡新城疫免疫效果的影响

The Influence of Wolfberry Polysaccharide on the Immune Effect of Chicks against Newcastle Disease Virus

为了研究枸杞多糖对雏鸡的免疫调节作用,将400羽1日龄雏鸡随机分为A、B、C、D共4组。7日龄时,分别给予A、B组雏鸡灌服10 mg/kg和20 mg/kg的枸杞多糖各5 m L,C、D组灌服同等剂量的生理盐水。给药的同时,A、B、C组滴鼻和点眼免疫新城疫Ⅳ系疫苗,D组不作处理。分别于7、14、21、28和35日龄时,采用微量法HI试验测定各组雏鸡新城疫抗体效价。分别于免疫后的第7、14、21、28和35天,计算各组雏鸡的免疫器官指数。试验结果显示,A、B组的新城疫抗体效价和各免疫器官指数明显高于C、D组,提示枸杞多糖对鸡新城疫疫苗的免疫效果具有明显的增强作用,且能够促进雏鸡中枢及外周免疫器官的发育。

In order to investigate the immunomodulatory effect of wolfberry polysaccharide on chicks, a total of 400 1 day old chicks were randomly divided into four groups （A, B, C, D）. When the chicks were 7 days old, the group A and B were gavaged with 5 mL of 10 mg/kg and 20 mg/kg of wolfberry polysacchafide respectively, the group C and D were gavaged with the same volume of saline. Meanwhile, the group A, B and C were vaccinated with Newcastle Disease virus IV strain vaccine by intraocular-nasal route, the group D were not vaccinated. The micro hemagglutination inhibition test was used to determine the antibody titer of blood serum of the four tested groups in 7, 14, 21, 28 and 35 days old, respectively, and the immune organ index of the four tested groups were calculated at the 7th, 14th, 21th, 28th and 35th day after vaccination, respectively. The results showed that the antibody titer of blood serum of the group A and B were higher than that of group C and D, indicating that the wolfberry polysaccharide could enhance the immune effect of chicks against Newcastle Disease virus.