猪硬脂酰辅酶A去饱和酶蛋白生物信息学分析

Bioinformatics Analysis of the SCD Protein in Pig

为了探究硬脂酰辅酶A去饱和酶(SCD)的结构和生物学功能,利用Expasy、Sopma等生物信息学软件,对猪SCD的氨基酸序列进行初步的生物信息学分析。结果表明:猪SCD基因编码359个氨基酸,平均分子质量为41.38 ku;该蛋白存在4个跨膜结构域,分别包含1个潜在的O-糖基化位点、3个N-糖基化位点和16个磷酸化位点;二级结构由α螺旋、β转角、延伸链和随机卷曲组成;该蛋白具有低复杂结构和跨膜结构两种功能结构域。

In order to explore the structure and biological iunction of SCD in pig, bioiniormatics software like Expasy and 3opma were used to analyze the amino acid sequence of SCD. The results showed that SCD protein of pig was composed by 359 amino acids, and had a predicted molecular weight of 41.38 ku. SCD had four transmembrane domains,respectively containing one potential O-glycosylation sites, three N-glycosylation sites and 16 phosphorylation sites. Alpha helix, beta turn, extended strand and random curl were the main components of secondary structure. The low complexity and transmembrance region were two functional structures of SCD.