液质联用法测定抗结核药物血药浓度

The detection of antituberculosis drugs concentration in plasma by HPLC-MS/MS

目的建立利用高效液相色谱质谱联用技术（HPLC-MS/MS）同时测定血浆中异烟肼（H）、利福平（R）、吡嗪酰胺（Z）、乙胺丁醇（E）4种药物浓度的方法。方法采用Waters Xterra C18色谱柱（3.5μm 2.1×150mm）;以0.01mol/L的乙酸铵：乙腈：甲醇（35∶10∶55）为流动相;流速0.2ml/min;柱温：室温;进样量为10μl。使用电喷雾离子源（ESI）,多反应离子监测（MRM）。结果 HRZE的线性范围分别为4.004-200.2ng/ml、20.06-1003ng/ml、20.12-1006ng/ml、0.8012-40.06ng/ml,检测限分别为1ng/ml、2ng/ml、2ng/ml、0.2ng/ml,RSD分别为1.74%、1.68%、1.46%、1.32%。结论该方法同时测定血浆中HRZE的血药浓度线性关系良好,结果稳定可靠。

Objective To establish a method for detecting the concentration of RFP,PZA,INH and EMB in plasma by HPLC-MS/MS simultaneously. Methods Column：Waters Xterra C18 （3.5μm 2.1×150mm） ;Mobile phase：0.01mol/L ammonium acetate ： acetonitrile ： methanol （ 35：10：55 ） ; Flow rate ： 0.2ml/min ; column temperature ： room temperature ; the injec- tion volume was lOlL. Electrospray ionization （ESI） , multiple reaction monitoring （MRM）. Results The linear ranges of HRZE were 4.004 - 200.2ng/ml,20.06 - 1003ng/ml,20. 12 - 1006ng/ml,0. 8012 - 40.06ng/ml, the detection limit were l ng/ml,2ng/ml,2ng/ml,0.2ng/ml, and RSD were 1.74%, 1.68%, 1.46%, 1.32%. Conclusion The method could be used to determine the content of HRZE in human plasma.