槲皮素-3-葡萄糖苷对大鼠脑缺血-再灌注损伤的保护作用

Protective effects of quercetin on-3-glucoside on cerebral ischemiareperfusion injury in rats

目的:观察槲皮素-3-葡萄糖苷(quercitrin-3-glucoside,缩写为QG)对脑缺血-再灌注损伤大鼠的保护作用。方法:采用线栓法制备大鼠脑缺血-再灌注损伤的动物模型。通过QG对脑缺血-再灌注大鼠神经功能评分,病理组织学变化以及脑细胞凋亡情况的影响,探讨QG对脑缺血-再灌注损伤大鼠的保护作用及其可能的作用机制。结果:QG剂量组神经功能评分较模型组显著降低(P<0.05);TUNEL阳性细胞数QG剂量组与模型组相比明显减少(P<0.05)。结论:槲皮素-3-葡萄糖苷可明显改善大鼠脑缺血-再灌注损伤,减轻缺血再灌注导致的脑水肿、氧化损伤和能量代谢障碍。

Objective：The aim of This paper is mainly to study the protective effects of-3- of quercetin glucoside （Quercitrin-3-glucoside, QG） on cerebral ischemia reperfusion in rats, concerning brain tissue and brain cell apoptosis in cerebral mtarctlon area. Methods： we prepare the MCAO model （using the suture method） 1 hour later. After 2 hours of ischemia , the rats received reperfusion administration for the following 24 hours. Then we determined the change of nerve function, infarct area, microscopic structure of brain tissue, and cell apoptosis in each group. Results： The changes of neurological function of rats： neural function score QG dose group was significantly lower than that in sham operation group（P〈0.05）; compared with the model group, the TUNEL positive cells decreased in control group and QG dose group（P〈0.05）. Conelusions：QG has a protective effect on cerebral ischemia reperfusion injury. The mechanism of this effect is probably by reducing oxidative damage and inhibiting apoptosis in cerebral ischemia reperfusion injury.