油田石油污染土壤微生物修复实验研究

Laboratory microbial remediation study of oil-contaminated soil in an oil field

为了有效预防和治理某油田因石油开发而造成的土壤污染，通过实验室选择性富集培养，从该油田石油污染土壤中筛选出了一组降解石油污染的优势菌群，开展石油污染土壤的微生物修复实验．在实验室条件下考察4种方法分别对高、中、低3种污染浓度的土壤样品的石油降解率的影响，确定出3种土壤样品的最佳处理方法．实验结果显示，3种土壤样品在最佳处理方法下，经过33d降解，其石油烃降解率可达37．93％-75．70％，说明本实验培养出的优势茵群能够对该油田的油污土壤进行修复，探索了该油田微生物修复技术应用的可行性．

In order to prevent and deal with the soil pollution caused by oil exploitation in an oil field, the effective degradation microbial communities, which were lab selective enrichment cultured from oil - contaminated soil of this oil field, was employed for microbial remediation experiments of oil - contaminated soil. Four methods affecting the degradation rate of oil for three soil samples of high, medium, low pollution concentration were observed under laboratory conditions, and the optimum processing methods for three soil samples were determined. The experimental results showed that the degradation rate of petroleum hydrocarbon for three soil samples in the optimal treatment method could reach 37.93% -75.70% after 33 d bioremediation, indicating that the effective degradation microbial communities, cultivated in this experiment, can remediate the oil- contaminated soil of this oil field. This paper has discussed the feasibility of application of microbial remediation technique in this oil field.