婴幼儿支原体感染与免疫功能的变化

Changes of immune function in infants with Mycoplasma pneumoniae infection

目的检测肺炎支原体(Mp)感染婴幼儿的细胞免疫功能和体液免疫功能,研究结果的变化规律,探讨临床相关的诊断指标。方法收集肺炎支原体感染的患儿的外周血标本,采用流式细胞术检测淋巴细胞表面分化抗原CD3、CD4、CD8的表达,利用生化仪检测血清中IgM、IgG、IgA的浓度;同时设立健康对照组。结果肺炎支原体患儿外周血CD3+T淋巴细胞、CD4+T淋巴细胞比例、CD4+/CD8+淋巴细胞比值和免疫球蛋白IgA、IgG浓度低于健康儿童,免疫球蛋白IgM浓度高于正常儿童;低拷贝组患儿外周血CD4+T淋巴细胞比例和免疫球蛋白IgM浓度低于高拷贝组。结论肺炎支原体感染的患儿免疫状态发生明显变化,T淋巴细胞免疫功能被抑制,体液免疫功能发生一定的紊乱。

Objective To detect the cellular immune and humoral immune function in infants with Mycoplasma pneumoniae（Mp） infection,and to study its change regularity and the related clinical indexes.Methods The peripheral blood samples were collected from infantile patients with MP infection.The expression of CD3,CD4 and CD8 on the surface of T lymphocyte（T-cell）were detec-ted by the flow cytometry and the concentrations of IgG,IgA and IgM in serum were detected by the automatic biochemistry analy-zer.At the same time the normal control group was set up.Results The proportion of CD3 ＋ and CD4 ＋ T-cell,CD4 ＋/CD8 ＋ ratio and the concentration of IgG,IgA in the patient group were significantly lower than those in the control group,while the concentra-tion of IgM in the patient group were significantly lower than those in the normal children.The concentration of IgM was higher than that in the normal children;the proportion of peripheral blood CD4 ＋ T-cell and the concentration of IgM in the low-copy group were significantly lower than those in the high-copy group.Conclusion The immune status in children with Mp infection has obvi-ous change.The immune function of T-cell is suppressed.the humoral immune function has certain turbulence.