应用高速离心技术消除乳糜干扰的关键参数研究

Research on key parameters of eliminating interference of chyle by high-speed centrifugation technique

目的探寻高速离心法消除乳糜干扰的关键离心参数。方法通过向本底血清中添加乳糜微粒建立乳糜血,在5个不同参数下,离心同一组模型,将其下层血清的生化指标与本底血清进行配对t检验,并对存在统计学差异的实验组进行相对偏倚分析。结果与本底血清相比,对于ALT、AST、TP、ALB、ALP、GGT、Fe 7项,相对离心力(RCF)29 703g离心20min时的相关系数较高,差异无统计学意义(P>0.01);对于CK、CHE 2项,20 672g离心15min时的相关系数较高,差异无统计学意义(P>0.01);对于Zn,9 168g离心5min时的相关系数较高,差异尚无统计学意义(P>0.01);对于Glu、LDH、CRE、Urea、UA、Ca、P7项所有实验组差异均具有统计学意义(P<0.01),但各组产生的相对偏倚均在可接受范围之内,对于Ca、P两项在9 168g离心5min时的相关系数较高。结论推荐使用29 703g离心20min消除乳糜对ALT、AST、TP、ALB、ALP、GGT、Fe、Glu、LDH、CRE、Urea、UA的干扰,使用20 672g离心15min消除对CK、CHE的干扰,使用9 168g离心5min消除对Zn、Ca、P的干扰。

Objective To explore the key parameters of eliminating the interference of chyle by adopting the high-speed centrifu-gation technique.Methods To establish the chylemia by adding chylomicrons to background serum,centrifuge the same model un-der five different parameters,compare the biochemical indicators of lower serum with background by matched t-test,and to analyze the relative bias of the experimental group with statistical differences.Results Compared with the background serum,for ALT, AST,TP,ALB,ALP,GGT and Fe,the correlation coefficient was higher and the differences had no statistical significance（P 〉0.01 ）when centrifuging for 20 min by RCF 29 703 g;for CK and CHE,the correlation coefficient was higher and the differences had no statistical significance（P 〉0.01）when centrifuging for 15 min by 20 672 g;for Zn,the correlation coefficient was higher and the differences had no statistical significance（P 〉0.01）when centrifuging for 5 min by 9 168 g.For Glu,LDH,CRE,Urea,UA,Ca and P,all the experimental groups had statistically significant differences（P 〈0.01）,but the relative bias in each group were within the acceptable range.For Ca and P,the correlation coefficient was higher when centrifuging for 15 min by 20 672 g.Conclusion It is recommended to eliminate the interference of chyle on ALT,AST,TP,ALB,ALP,GGT,Fe,Glu,LDH,CRE,Urea and UA by u-sing the centrifugal parameters（29 703 g,20 min）,on CK and CHE by using the centrifugal parameters（20 672 g,15 minutes）and on Zn,Ca and P by using the centrifugal parameters（9 168 g,5 minutes）.