痰标本涂片对痰培养的指导意义

Guidance significance of sputum smear on sputum culture

目的对227例痰标本涂片镜检与培养结果进行对比,了解痰涂片对痰培养的指导意义。方法收集227例痰标本,观察性状,涂片染色镜检,根据标准分为A、B、C、D 4类,同时进行培养,分析涂片和培养的关系。结果 227例标本中合格痰标本158例,占69.60%,不合格标本69例,占30.40%。227例痰标本128例检出潜在病原菌,阳性检出率为56.39%,合格标本培养阳性率为70.39%,不合格标本培养阳性率为28.00%。合格痰标本涂片结果与培养结果的符合率为72.78%。合格痰标本中细胞吞噬菌的阳性率为11.72%,其中合格A类痰标本细胞吞噬菌14例,阳性率为16.67%,B类痰标本细胞吞噬菌5例,阳性率为6.76%。结论痰标本涂片镜检能为呼吸道感染的诊断和治疗提供重要信息,痰标本涂片对痰培养有重要的指导意义。

Objective To understand the guidance significance of sputum smear on the sputum culture by comparing the micro-scopic detection results of sputum smear with the sputum culture results in 227 cases.Methods 227 cases of sputum specimen were collected for observing the character and smear staining by microscopy.These specimens were divided into the class A,B,C and D according to the standards and simultaneously conducted the culture.The relationship between smear and culture was analyzed.Reˉ sults Among 227 cases of sputum specimen,158 cases were qualified and accounted for 69.60%,and 69 cases were unqualified and accounted for 30.40%.Potential pathogens were detected in 128 cases,the positive detection rate was 56.39%,the positive detec-tion rate of qualified sputum specimens was 70.39% and which of unqualified sputum specimens was 28.00%.The coincidence rate of qualified sputum specimen smear results and the culture results was 72.78%.The positive rate of cytophagic bacteria in the qual-ified sputum specimens was 11.72%,in which 14 cases of cytophagic bacteria were the qualified class A sputum specimens with the positive rate of 16.67% and 5 cases of cytophagic bacteria were the class B sputum specimens with the positive rate of 6.76 %. Conclusion The sputum smear microscopic detection can provide the important information for the diagnosis and treatment of re-spiratory infections and the sputum smear has the important guiding significance for sputum culture.