滤蓝光变色人工晶体对兔视网膜光损伤保护作用的实验研究

Experimental study on the protective effect of a blue light-filtering intraocular lens from retinal injury

目的探讨短波长蓝光对兔视网膜造成损伤的可能机制及滤蓝光变色人工晶体对兔视网膜色素上皮细胞的保护功能。方法将健康新西兰兔26只随机分为两组,分别在行晶体摘除术后植入滤蓝光变色人工晶体和普通紫外线阻断型人工晶体,后用UVR光源(350-450nm)每天照射实验兔眼8h,3个月后取视网膜组织制成冰冻切片后行免疫组化法检测血管生长因子(VEGF)的表达,TUNEL法检测黄斑区视网膜色素上皮层细胞(RPE细胞)凋亡情况。结果 UVR照射后滤蓝光变色人工晶体较普通紫外线阻断型人工晶体黄斑区视网膜RPE细胞出现凋亡改变减少,免疫组化法检测植入普通紫外线阻断型人工晶体组VEGF的表达升高(P<0.05)。结论短波长蓝光能导致兔视网膜RPE细胞凋亡,功能障碍,同时刺激VEGF表达增多,导致眼底视网膜病变。滤蓝光变色人工晶体能有效阻断紫外蓝光,对视网膜细胞有保护作用。

Objective To explore the blue light-filtering intraocular lens （IOLs） protection on retinal pigment epithelium（RPE）. Methods 26 healthy rabbits with phacoemulsification were randomly divided into two groups,Half were implanted the blue light-filtering IOLs,the other half were implanted the ultraviolet （UV） blocking IOLs,with ultraviolet light （UVR） daily irradiation experi-ments in rabbit eyes 8 hours a day. After 3 months retinal tissue biopsy were carried out by H-E staining ,retinal tissue frozen sections underwent immunohistochemical to evaluate expression of vascular endothelial growth factor （VEGF）assay ,apoptosis by TUNEL of macular retinal conditions. Results After UVR irradiation increased retinal RPE cells apoptosis were found in the rab-bits with the ultraviolet （UV） blocking IOLs,more than the rabbits with blue light-filtering IOLs. Immunohistochemical assay ele-vated VEGF expression obviously increased in ultraviolet blocking IOLs group compare to the blue light-filtering IOLs group. （P〈0.05）. Conclusion The blue light-filtering IOLs has a protective effect on retinal cells for it can block the blue light inducing apoptosis of retinal RPE cells,sitimulating VEGF expression and causing dysfunction of retinal.