金钗石斛水煎剂对糖尿病肾病大鼠肾脏PPARγ表达的影响

Effect of Dendrobium Nobile Lindl Decoction on Expression of PPARγ in Diabetic Nephropathy Rat Kidney

目的通过观察金钗石斛水煎剂对糖尿病肾病(DN)大鼠肾皮质过氧化物酶体增殖物激活受体γ(PPARγ)表达的影响,探讨其对DN的保护作用及相关机制。方法将60只健康SD大鼠随机分为6组,每组10只:正常对照组(NC组),模型对照组(DN组),罗格列酮组(RGZ组),金钗石斛小剂量(LD)、中剂量(MD)、大剂量(HD)干预组。在大鼠糖尿病造模成功后各组分别给药12周,观察大鼠肾脏病理、Real-time PCR法检测大鼠肾皮质PPARγmRNA表达、Western blot法检测大鼠肾皮质中PPARγ蛋白表达的情况。结果金钗石斛水煎剂能明显改善糖尿病大鼠肾脏基底膜增厚及足突增粗融合;增加DN大鼠肾皮质中PPARγmRNA及蛋白的表达(P<0.05或P<0.01)。结论金钗石斛水煎剂可以减轻DN大鼠的肾损伤,其机制可能与上调DN大鼠肾皮质中PPARγ表达有关。

Objective To explore the protective mechanism of Dendrobium nobile lindl（ DNL） decoction on the expression of peroxisome proliferator activated receptor gamma（ PPARγ） in the renal cortex of diabetic nephropathy（ DN） rats.Methods Sixty SD rats were randomly divided into six groups（ n = 10 per group） as follows： normal control group（ NC）,diabetic nephropathy control group（ DN）,rosiglitazone group（ RGZ）,the Dendrobium nobile lindl low（ LD）,medium（ MD） and high（ HD） dose groups. After DN rat model was established,the rats were administrated with respective medications for 12 weeks,respectively. The renal pathology of rats was observed. The mRNA and protein expression of PPARγ in the renal cortex were detected via Real-time PCR and Western blotting,respectively. Results High dose DNL decoction significantly alleviated thickening of kidney tissue basement membrane and fusion of foot process in model rats. The levels of PPARγ mRNA and protein expression in the MD and HD groups were significantly increased as compared with the DN group（ P〈0. 05,P〈0. 01）.Conclusion DNL decoction can effectively reduce kidney injury by up-regulating the PPARγ mRNA and protein expression in diabetic nephropathy rats.