Stat3调控MMP9和TIMP1的转录与表达

Regulating the Transcription and Expression of Matrix Metalloproteinase-9(MMP9) and the Tissue Inhibitor of Metalloproteinase 1 (TIMP1) with Stat3

通过脂质体瞬时转染Stat3重组质粒的方法在NIH3T3细胞中过表达Stat3;采用RT-PCR,Western blot以及荧光素酶活性分析的方法评估Stat3对MMP9及对其内源性抑制因子TIMP1的影响.结果表明:Stat3可增强MMP9及TIMP1基因启动子的活性,促进两者的转录与表达;并且Stat3对TIMP1的促进作用明显强于对MMP9的促进作用,从而使ECM的降解能力减弱,细胞外基质成分积累,导致纤维化的发生.

Gene Stat3 was overexpressed in NIH3T3 cells by lipofection transfection,and the effect of Stat3 on the expres-sion of MMP9 and its inhibitor TIMP1 was estimated by RT-PCR,Western Blot and Luciferase Assay. The results show that Stat3 is able to upregulate the transcription and expression of MMP9 and TIMP1 by enhancing their promoter activities and the enhancement of TIMP1 expression induced by Stat3 is stronger than MMP9. It suggests that overexpression of Stat3 may inhibit the degradation of ECM in NIH3T3 cells and promote the development of fibrosis.