猴面花miRNA及靶基因的生物信息学预测

Bioinformatic Prediction of microRNA and Their Target Gene in Mimulus gutatus

以猴面花基因组序列为试材,通过psRobot网站进行茎环结构预测,应用blast-2.2.27+软件与rfam数据库和pfam数据库比对后去除非miRNA序列,应用bioedit分析miRNA序列及前体序列的碱基组成特点。结果表明:猴面花基因组中共发现105条成熟miRNA序列,分别属于49个不同miRNA家族;miRNA序列和其侧翼序列都存在碱基偏倚现象,miRNA5′端第1碱基和第19碱基尿嘧啶和胞嘧啶出现的频率分别为67.6%和49.5%;93个miRNA预测到了靶基因,其中有64个靶向转录因子。

Taking genome sequence of M.gutatus as material,prediction of stem loop structure of premiRNAs was run on the web-based software psRobot,then non-miRNA sequences were removed by BLAST search against rfam and pfam database.The characteristics of base composition was analyzed by bioedit software in miRNA sequences and their precursors.The result showed that there are 105 mature sequences in M.gutatus which belong to 49 different miRNA familes.The base bias phenomenon has been found in miRNA sequence and their flanking sequences.The frequency of uracil and cytosine is respectively67.6% and 49.5%in the first base and nineteenth base from miRNA 5′end.The target genes has been found in 93 miRNAs,in which 64 miRNAs regulate transcription factors.