骨髓间充质干细胞联合电针对大鼠糖尿病周围神经病神经营养因子表达的影响

Effect of Electroacupuncture Combined with Bone Marrow Mesenchymal Stem Cells on Neurotrophic Factors of Rat Models with Diabetic Peripheral Neuropathy

目的:探讨骨髓间充质干细胞(BMSC)联合电针对大鼠糖尿病周围神经病(DPN)坐骨神经中神经生长因子(NGF)和神经营养因子3(NT-3)表达的影响。方法:链脲佐菌素诱导制备DPN大鼠模型,成年大鼠随机分为模型组、电针组、BMSC组、电针加BMSC组。电针组取肝俞、脾俞、肾俞、足三里行电针治疗。BMSC组尾静脉注射BMSC细胞悬液,电针加BMSC组联合电针和BMSC移植治疗。造模14天后行坐骨神经传导速度检测,实时荧光定量PCR检测坐骨神经中NGF和NT-3 mRNA表达。结果:与模型组比较,电针组、BMSC组和电针加BMSC组坐骨神经传导速度均显著增高(P<0.01),坐骨神经中NGF和NT-3 mRNA表达显著增加(P<0.01)。其中电针加BMSC组较电针组和BMSC组神经传导速度改善更为显著,坐骨神经NGF和NT-3 mRNA表达显著增高(P<0.05)。结论:骨髓间充质干细胞联合电针促进大鼠糖尿病周围神经病神经功能恢复作用优于单独治疗组,其机制可能与联合治疗协同促进NGF和NT-3等神经营养因子表达有关。

Objective：To investigate the effect of electroacupuncture（ EA） combined with bone marrow mesen-chymal stem cells （ BMSC） transplanted on the expression of nerve growth factor （ NGF） and neurotrophins-3 （NT-3） in the sciatic nerve of rats with diabetic peripheral neuropathy（DPN）.Methods：The rat models with diabetic peripheral neuropathy were established by streptozotocin peritoneal injection method.Healthy SD rats were randomly divided into a model group,an EA group,a BMSC group and an EA＋BMSC group.EA treat-ment was applied to“Ganyu”,“Piyu” ,“Shenyu” and“Zusanli” in EA group;BMSC suspension was infused into the caudal vein in BMSC group;EA＋BMSC group was combined EA with BMSC.14d after modeling, the conduction velocity of sciatic nerve was measured,and the mRNA expressions of NGF and NT-3 in the sciatic nerve were detected by RT-PCR.Results：Compared with the model group, the conduction velocity of sciatic nerve was significantly increased in EA group, BMSC group and EA＋BMSC group （P〈0.01）,and the mR-NA expressions of NGF, NT-3 in sciatic nerve were significantly increased （P〈0.01）.Compared with EA group and BMSC group, the conduction velocity was significantly increased in EA＋BMSC group, and the ex-pressions of NGF and NT-3 were increased （P〈0.05）.Conclusion：The combined treatments promote con- 〈br〉 duction velocity of sciatic nerve more significantly than either of the single treatment in DPN rats , which may be related to the combined treatments which have synergistic effect on increasing the expressions of NGF and NT-3 .