香苇二石口服液对大鼠试验性热应激的影响

Effect of Herba Elsholtziae and Agastachis on the heat stress in rats

本试验通过检测大鼠肠道中热休克蛋白基因的表达,探究不同剂量香苇二石口服液对热应激的预防和治疗作用。45只健康雄性大鼠随机分为对照组、热应激组、自然恢复组、高剂量预防组、中剂量预防组、低剂量预防组、高剂量治疗组、中剂量治疗组、低剂量治疗组,建立高温应激模型,利用Real time-PCR技术检测大鼠小肠中HSP27、HSP70、HSP90 m RNA的表达变化。结果表明,高温刺激能极显著增加HSPs m RNA的表达量(P<0.01);香苇二石口服液能显著(P<0.05)和极显著(P<0.01)抑制HSPs m RNA的高表达。表明香苇二石口服液高剂量有极显著预防作用,中剂量有极显著治疗作用。

The pro-pose of this study was to investigate the potential protective effect and mechanisms of Chinese herbal compound on the expression of HSPs induced by heat stress. In this compound, Herba Elsholtziae and Herba Agastaehe were the main components. In this study, 45 rats were divided into control, heat-stress, prevention and treatment groups. Fresh extracts were prepared right before the experiment. Three concentrations of extract were prepared for high, medium and low concentrations, respectively.The result showed that heat stress significantly increased mRNA expression of HSPs（P〈0.01 ） ; Chinese herbal compound could significantly suppress the high expression induced by high temperature. The conclusion is that this compound is effective to resist heat stress by medial-dose in prevention and high-dose in treatment group.